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  • Featured Product
  • KD/KO Validated

MYH9 Polyclonal antibody

MYH9 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11128-1-AP

Synonyms

MYH9, Myosin 9, Myosin heavy chain 9, myosin IIa, Nonmuscle myosin IIa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYH9 Polyclonal antibody (11128-1-AP)

    WB analysis using 11128-1-AP

    Various cell lysates were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MYH9 Polyclonal antibody (11128-1-AP)

    WB analysis of human brain using 11128-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MYH9 Polyclonal antibody (11128-1-AP)

    WB analysis of mouse brain using 11128-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using MYH9 Polyclonal antibody (11128-1-AP)

    WB analysis of mouse kidney using 11128-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using MYH9 Polyclonal antibody (11128-1-AP)

    WB analysis of mouse colon using 11128-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MYH9 Polyclonal antibody (11128-1-AP)

    IP experiment of mouse brain using 11128-1-AP

    IP result of anti-MYH9 (IP:11128-1-AP, 5ug; Detection:11128-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MYH9 Polyclonal antibody (11128-1-AP)

    IHC staining of human lung cancer using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MYH9 Polyclonal antibody (11128-1-AP)

    IHC staining of human lung cancer using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MYH9 Polyclonal antibody (11128-1-AP)

    IHC staining of mouse kidney using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MYH9 Polyclonal antibody (11128-1-AP)

    IHC staining of mouse kidney using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYH9 Polyclonal antibody (11128-1-AP)

    IHC staining of human kidney using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11128-1-AP (MYH9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH9 Polyclonal antibody (11128-1-AP)

    IF Staining of HepG2 using 11128-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using MYH9 Polyclonal antibody (11128-1-AP)

    IF Staining of mouse small intestine using 11128-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH9 Polyclonal antibody (11128-1-AP)

    IF Staining of HepG2 using 11128-1-AP

    Immunofluorescent analysis of HepG2 cells, using MYH9 antibody 11128-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Flow cytometry (FC) experiment of HepG2 cells using MYH9 Polyclonal antibody (11128-1-AP)

    FC experiment of HepG2 using 11128-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human MYH9 (11128-1-AP) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) at dilution 1:200 (red), or 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, mouse kidney tissue, mouse colon tissue, human brain tissue, U2OS cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse small intestine tissue, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11128-1-AP targets MYH9 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYH9 fusion protein Ag1612
Full Name myosin, heavy chain 9, non-muscle
Calculated Molecular Weight 227 kDa
Observed Molecular Weight 224 kDa
GenBank Accession NumberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
RRIDAB_2147294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Protocols

Product Specific Protocols
WB protocol for MYH9 antibody 11128-1-APDownload protocol
IHC protocol for MYH9 antibody 11128-1-APDownload protocol
IF protocol for MYH9 antibody 11128-1-APDownload protocol
IP protocol for MYH9 antibody 11128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Acta Pharm Sin B

Ginsenoside Rg1 ameliorates blood-brain barrier disruption and traumatic brain injury via attenuating macrophages derived exosomes miR-21 release.

Authors - Kefeng Zhai
View Article
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
View Article
humanWB,IF

Cell Death Differ

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling

Authors - Shunfeng Hu
  • KD Validated
View Article
human,mouseWB,IHC,IF

Cell Rep

Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells

Authors - Yingqi Liu
View Article
mouseIF,CoIP,WB

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
View Article
mouseIF,WB

Stem Cell Reports

Non-muscle myosin heavy chain 9 maintains intestinal homeostasis by preventing epithelium necroptosis and colitis adenoma formation.

Authors - Shan Wang
  • KO Validated
View Article