MYH9 Polyclonal antibody
MYH9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11128-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, mouse colon tissue, mouse kidney tissue, mouse brain tissue, human brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human lung cancer tissue, human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:3000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 6 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
CoIP | See 2 publications below |
Product Information
11128-1-AP targets MYH9 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MYH9 fusion protein Ag1612 |
Full Name | myosin, heavy chain 9, non-muscle |
Calculated molecular weight | 227 kDa |
Observed molecular weight | 224 kDa |
GenBank accession number | BC011915 |
Gene symbol | MYH9 |
Gene ID (NCBI) | 4627 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MYH9 antibody 11128-1-AP | Download protocol |
IHC protocol for MYH9 antibody 11128-1-AP | Download protocol |
IF protocol for MYH9 antibody 11128-1-AP | Download protocol |
IP protocol for MYH9 antibody 11128-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Invest Ophthalmol Vis Sci SWATH-MS Proteomic Analysis of Oxygen-Induced Retinopathy Reveals Novel Potential Therapeutic Targets. | ||
Cell Death Dis NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90. | ||
Oncol Lett MYH9 overexpression correlates with clinicopathological parameters and poor prognosis of epithelial ovarian cancer. | ||
Reproduction Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion. | ||
Exp Cell Res The Actomyosin Network is Influenced by NMHC IIA and Regulated by CrpF46, which is Involved in Controlling Cell Migration. | ||
Am J Pathol S100A4/Nonmuscle Myosin IIA/p53 Axis Contributes to Aggressive Features in Ovarian High-Grade Serous Carcinoma. |