MYH9 Polyclonal antibody
MYH9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11128-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse colon tissue, mouse kidney tissue, mouse brain tissue, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue, human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 6 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
11128-1-AP targets MYH9 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYH9 fusion protein Ag1612 |
| Full Name | myosin, heavy chain 9, non-muscle |
| Calculated molecular weight | 227 kDa |
| Observed molecular weight | 224 kDa |
| GenBank accession number | BC011915 |
| Gene symbol | MYH9 |
| Gene ID (NCBI) | 4627 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH9 antibody 11128-1-AP | Download protocol |
| IHC protocol for MYH9 antibody 11128-1-AP | Download protocol |
| IF protocol for MYH9 antibody 11128-1-AP | Download protocol |
| IP protocol for MYH9 antibody 11128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci SWATH-MS Proteomic Analysis of Oxygen-Induced Retinopathy Reveals Novel Potential Therapeutic Targets. | ||
Cell Death Dis NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90. | ||
Oncol Lett MYH9 overexpression correlates with clinicopathological parameters and poor prognosis of epithelial ovarian cancer. | ||
Reproduction Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion. | ||
Exp Cell Res The Actomyosin Network is Influenced by NMHC IIA and Regulated by CrpF46, which is Involved in Controlling Cell Migration. | ||
Am J Pathol S100A4/Nonmuscle Myosin IIA/p53 Axis Contributes to Aggressive Features in Ovarian High-Grade Serous Carcinoma. |
