|Positive WB detected in||A549 cells, mouse colon tissue, mouse kidney tissue, mouse brain tissue, human brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human lung cancer tissue, human kidney tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:3000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11128-1-AP targets MYH9 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MYH9 fusion protein Ag1612|
|Full Name||myosin, heavy chain 9, non-muscle|
|Calculated molecular weight||227 kDa|
|Observed molecular weight||224 kDa|
|GenBank accession number||BC011915|
|Gene ID (NCBI)||4627|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
|Product Specific Protocols|
|WB protocol for MYH9 antibody 11128-1-AP||Download protocol|
|IHC protocol for MYH9 antibody 11128-1-AP||Download protocol|
|IF protocol for MYH9 antibody 11128-1-AP||Download protocol|
|IP protocol for MYH9 antibody 11128-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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