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MYH9 Polyclonal antibody

MYH9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 11128-1-AP

Publications (82)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA

Synonyms

Myosin 9, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Myosin-9, NMMHC II-a

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 11128-1-AP
Various cell lysates were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 11128-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse kidney using 11128-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse colon using 11128-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 11128-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse brain using 11128-1-AP
IP result of anti-MYH9 (IP:11128-1-AP, 5ug; Detection:11128-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11128-1-AP (MYH9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11128-1-AP (MYH9 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse small intestine using 11128-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11128-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U2OS using 11128-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MYH9 antibody (11128-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11128-1-AP
Immunofluorescent analysis of HepG2 cells, using MYH9 antibody 11128-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HepG2 using 11128-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MYH9 Polyclonal antibody (11128-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (82)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, mouse brain tissue, mouse kidney tissue, mouse colon tissue, human brain tissue, U2OS cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lung cancer tissue, human kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse small intestine tissue
Positive IF/ICC detected in	HepG2 cells, U2OS cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11128-1-AP targets MYH9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1612 Product name: Recombinant human MYH9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1741-1960 aa of BC011915 Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE Predict reactive species
Full Name	myosin, heavy chain 9, non-muscle
Calculated Molecular Weight	227 kDa
Observed Molecular Weight	224 kDa
GenBank Accession Number	BC011915
Gene Symbol	MYH9
Gene ID (NCBI)	4627
RRID	AB_2147294
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35579
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Protocols

Product Specific Protocols
FC protocol for MYH9 antibody 11128-1-AP	Download protocol
IF protocol for MYH9 antibody 11128-1-AP	Download protocol
IHC protocol for MYH9 antibody 11128-1-AP	Download protocol
IP protocol for MYH9 antibody 11128-1-AP	Download protocol
WB protocol for MYH9 antibody 11128-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-MYH9 antibody (Cat# 11128-1-AP) has 82 citations across high-impact journals including Nature, Cell, Nat Commun, Sci Adv, Cell Death Differ, Oncogene, and Cancer Lett. Research fields span oncology (HCC, gastric, ovarian, lung, pancreatic cancer), cardiovascular disease, neuroscience, immunology/inflammation, virology, cytoskeleton biology, and metabolism. Applications include WB, IF, IHC, CoIP, and IP.

Species	Application	Title
human	WB,IP	Signal Transduct Target Ther The p-MYH9/USP22/HIF-1α axis promotes lenvatinib resistance and cancer stemness in hepatocellular carcinoma Authors - Qiaonan Shan View Article
human	WB,IP,IF	Nature TMEFF1 is a neuron-specific restriction factor for herpes simplex virus Authors - Yao Dai KO Validated View Article
human	WB,CoIP	Mol Cancer OLFM4 promotes the progression of intestinal metaplasia through activation of the MYH9/GSK3β/β-catenin pathway Authors - Hongfa Wei View Article
	IF	Nat Biomed Eng PIEZO1 mechanically regulates the antitumour cytotoxicity of T lymphocytes Authors - Ruiyang Pang View Article
human	WB	Nat Commun Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation Authors - Siyuan Sun View Article
		Nat Commun RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis. Authors - Tong Wu View Article