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Mcp1 Antibody 12 Publications

Mouse Monoclonal| Catalog number: 66272-1-Ig |CloneNo.: 1B9F7

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Con: 200 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Mcp1 Antibody IHC mouse kidney tissue 66272-1-Ig

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig( Mcp1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Mcp1 Antibody IHC mouse kidney tissue 66272-1-Ig

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig( Mcp1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Mcp1 Antibody WB LPS treated RAW 264.7 cells 66272-1-Ig

LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66272-1-Ig( Mcp1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
mouse, rabbit, rat
Positive WB detected in:
LPS treated RAW 264.7 cells
Positive IHC detected in:
mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Mcp1 fusion protein Ag24085
Full name:
chemokine (C-C motif) ligand 2
Calculated molecular weight:
Observed molecular weight:
16 kDa
GenBank accession number:
NM-011333
Gene ID (NCBI):
20296
Gene symbol
Ccl2
Synonyms
AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF

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