Mcp1 Antibody

Name: Mcp1 Mouse Monoclonal antibody
Brand: Proteintech
SKU: 66272-1-Ig

Mcp1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1B9F7

Review Product

Cat no : 66272-1-Ig

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF

Validation Data Gallery

WB analysis of DC2.4 cells using 66272-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours
IHC staining of mouse kidney tissue using 66272-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig( Mcp1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
IHC staining of mouse kidney tissue using 66272-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig( Mcp1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

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Cat No. 66272-1-Ig

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Tested Applications

Positive WB detected in	DC2.4 cells, LPS treated RAW 264.7 cells, nouse lung tissue, mouse brain tissue
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 19 publications below
IHC	See 5 publications below
IF	See 2 publications below

Product Information

66272-1-Ig targets Mcp1 in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.

Reactivity	mouse
Cited Species	mouse, rabbit, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Mcp1 fusion protein Ag24085
Full Name	chemokine (C-C motif) ligand 2
Calculated molecular weight
Observed molecular weight	26 kDa
GenBank accession number	NM-011333
Gene symbol	Ccl2
Gene ID (NCBI)	20296
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Mcp1 antibody 66272-1-Ig	Download protocol
IHC protocol for Mcp1 antibody 66272-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Biomed Pharmacother Administration of methyl palmitate prevents non-alcoholic steatohepatitis (NASH) by induction of PPAR-α. Authors - Li Zhang View Article
mouse	IF	Theranostics Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice. Authors - Xinzhi Li View Article
mouse	WB	Osteoarthritis Cartilage Clock mutant promotes osteoarthritis by inhibiting the acetylation of NFκB. Authors - G Yuan View Article
rat	WB	Regen Med Autologous nanofat transplantation accelerates foot wound healing in diabetic rats. Authors - Li Chen View Article
mouse	WB	Biomed Pharmacother Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2. Authors - Fanchao Feng View Article
rat	WB	Evid Based Complement Alternat Med Louqin Zhisou Decoction Inhibits Mucus Hypersecretion for Acute Exacerbation of Chronic Obstructive Pulmonary Disease Rats by Suppressing EGFR-PI3K-AKT Signaling Pathway and Restoring Th17/Treg Balance. Authors - Feng Feng View Article

Mcp1 Antibody

Mcp1 Monoclonal Antibody for IHC, WB, ELISA

Validation Data Gallery

WB analysis of DC2.4 cells using 66272-1-Ig

IHC staining of mouse kidney tissue using 66272-1-Ig

IHC staining of mouse kidney tissue using 66272-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Administration of methyl palmitate prevents non-alcoholic steatohepatitis (NASH) by induction of PPAR-α.

Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice.

Clock mutant promotes osteoarthritis by inhibiting the acetylation of NFκB.

Autologous nanofat transplantation accelerates foot wound healing in diabetic rats.

Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2.

Louqin Zhisou Decoction Inhibits Mucus Hypersecretion for Acute Exacerbation of Chronic Obstructive Pulmonary Disease Rats by Suppressing EGFR-PI3K-AKT Signaling Pathway and Restoring Th17/Treg Balance.