NAPRT1 Polyclonal antibody

NAPRT1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13549-1-AP

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856



Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, mouse kidney tissue, human colon tissue, L02 cells, HEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13549-1-AP targets NAPRT1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAPRT1 fusion protein Ag4265
Full Name nicotinate phosphoribosyltransferase domain containing 1
Calculated molecular weight 514 aa, 55 kDa
Observed molecular weight 51 kDa, 56 kDa
GenBank accession numberBC032466
Gene symbol NAPRT1
Gene ID (NCBI) 93100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Protocols

Product Specific Protocols
WB protocol for NAPRT1 antibody 13549-1-APDownload protocol
IP protocol for NAPRT1 antibody 13549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Metab

CD38 is methylated in prostate cancer and regulates extracellular NAD.

Authors - Jack Mottahedeh
humanWB,IHC

Front Oncol

NAMPT and NAPRT, Key Enzymes in NAD Salvage Synthesis Pathway, Are of Negative Prognostic Value in Colorectal Cancer.

Authors - Xiao-Qin Li
humanWB,IHC

APMIS

Expression patterns of nicotinamide phosphoribosyltransferase and nicotinic acid phosphoribosyltransferase in human malignant lymphomas.

Authors - Olesen Uffe Høgh UH
humanWB

J Biol Chem

Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.

Authors - Cerna David D
humanWB,IHC

Mol Cell Biol

The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.

Authors - Watson Mark M
humanWB

Mol Cancer Ther

Dual and specific inhibition of NAMPT and PAK4 by KPT-9274 decreases kidney cancer growth.

Authors - Omran Abu Aboud