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CoraLite®488-conjugated NAPRT1 Monoclonal antibody

NAPRT1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

5D8H10

Cat no : CL488-66159

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66159-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66159 targets NAPRT1 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NAPRT1 fusion protein Ag4265
Full Name nicotinate phosphoribosyltransferase domain containing 1
Calculated molecular weight 514 aa, 55 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC032466
Gene symbol NAPRT1
Gene ID (NCBI) 93100
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Protocols

Product Specific Protocols
IF protocol for CL488 NAPRT1 antibody CL488-66159Download protocol
Standard Protocols
Click here to view our Standard Protocols