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  • KD/KO Validated

NEDD4L Antibody

NEDD4L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 13690-1-AP

Synonyms

hNedd4 2, KIAA0439, NEDD4 2, NEDD4.2, NEDD4L, NEDL3, RSP5



Tested Applications

Positive WB detected inC6 cells, HT-1080 cells, PC-3 cells
Positive IP detected inC6 cells
Positive IHC detected inhuman prostate cancer tissue, human stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13690-1-AP targets NEDD4L in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD4L fusion protein Ag4565
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Calculated molecular weight 854aa,96 kDa
Observed molecular weight 112 kDa, 125 kDa
GenBank accession number BC032597
Gene symbol NEDD4L
Gene ID (NCBI) 23327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction(PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation(PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Protocols

Product Specific Protocols
WB protocol for NEDD4L antibody 13690-1-APDownload protocol
IHC protocol for NEDD4L antibody 13690-1-APDownload protocol
IF protocol for NEDD4L antibody 13690-1-APDownload protocol
IP protocol for NEDD4L antibody 13690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Exp Pathol

Pathobiological properties of the ubiquitin ligase Nedd4L in melanoma.

Authors - Yusuke Kito
  • KD Validated
humanWB,IHC

Int J Exp Pathol

Pathobiological properties of the ubiquitin ligase Nedd4L in melanoma.

Authors - Yusuke Kito
  • KD Validated
humanWB,IHC

Int J Exp Pathol

Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer.

Authors - Takeuchi Tamotsu T
humanWB,IHC

Int J Exp Pathol

Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer.

Authors - Takeuchi Tamotsu T
humanIHC

Med Oncol

Decreased expression of Nedd4L correlates with poor prognosis in gastric cancer patient.

Authors - Gao Chengcheng C
humanWB,IHC

Int J Med Sci

The correlation between NEDD4L and HIF-1α levels as a gastric cancer prognostic marker.

Authors - Xingwang Jiang