Tested Applications
| Positive WB detected in | SH-SY5Y cells, Neuro-2a cells, mouse brain tissue, C6 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12998-1-AP targets NF-L in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3667 Product name: Recombinant human NEFL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC039237 Sequence: MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTIN Predict reactive species |
| Full Name | neurofilament, light polypeptide |
| Calculated Molecular Weight | 543 aa, 62 kDa |
| Observed Molecular Weight | 65-68 kDa, 140-160 kDa |
| GenBank Accession Number | BC039237 |
| Gene Symbol | NF-L |
| Gene ID (NCBI) | 4747 |
| RRID | AB_10597388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07196 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NF-L antibody 12998-1-AP | Download protocol |
| IF protocol for NF-L antibody 12998-1-AP | Download protocol |
| IHC protocol for NF-L antibody 12998-1-AP | Download protocol |
| IP protocol for NF-L antibody 12998-1-AP | Download protocol |
| WB protocol for NF-L antibody 12998-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NF-L Polyclonal antibody (Cat# 12998-1-AP) has accumulated 36 citations across journals including Nature Neuroscience, ACS Nano, Molecular Cell, Advanced Science, Journal of Neuroinflammation, Materials Today Bio, Environmental International, Journal of Translational Medicine, Cell Reports, and Analytical Chemistry. Associated research fields encompass neuroscience (neurodegeneration, traumatic brain injury, spinal cord injury, epilepsy), neural regeneration, neuroinflammation, cancer biology, biomarker discovery, and tissue engineering.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Lipid-accumulated reactive astrocytes promote disease progression in epilepsy | ||
ACS Nano Microarray Chip-Based High-Throughput Screening of Neurofilament Light Chain Self-Assembling Peptide for Noninvasive Monitoring of Alzheimer's Disease | ||
Mol Cell Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury. | ||
Adv Sci (Weinh) An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress. | ||
J Neuroinflammation Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-κB pathway in LPC-induced demyelination | ||
Mater Today Bio In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation. |
Reviews
What customers say
The single user review for 12998-1-AP is a 5-star rating from Max Holzer (February 2022). He used the antibody at a 1:2000 dilution for Western Blot on hamster brain lysate (lot 00003769) and reported strong and specific labeling of NfL. Overall, the feedback is positive, confirming good performance and specificity in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Max (Verified Customer) (02-24-2022) | strong and specific labeling of NfL in hamster brain lysate on western blot
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