NF-M-Specific Polyclonal antibody

NF-M-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20664-1-AP

Synonyms

NEF3, NEFM, neurofilament, NF, NF M, NFM



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, human brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20664-1-AP targets NF-M-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name neurofilament, medium polypeptide
Calculated molecular weight 102 kDa
Observed molecular weight 140-160 kDa
GenBank accession numberNM_005382
Gene symbol NEFM
Gene ID (NCBI) 4741
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. The antibody is specific to NEFM.

Protocols

Product Specific Protocols
WB protocol for NF-M-Specific antibody 20664-1-APDownload protocol
IHC protocol for NF-M-Specific antibody 20664-1-APDownload protocol
IF protocol for NF-M-Specific antibody 20664-1-APDownload protocol
IP protocol for NF-M-Specific antibody 20664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols