A431 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HL-60 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10268-1-AP( IkB alpha antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP( IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

pig liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP( IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

Immunohistochemical of paraffin-embedded human ovary tumor using 10268-1-AP(IkB alpha antibody) at dilution of 1:100 (under 25x lens)

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

Immunohistochemical of paraffin-embedded human prostate cancer using 10268-1-AP(IkB alpha antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human prostate cancer using 10268-1-AP(IkB alpha antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human stomach tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human stomach tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

IP Result of anti-IkB alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:1000) with HeLa cells lysate 4000ug.

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 10268-1-AP(IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 HeLa cells were stained with 0.2ug IkB alpha antibody (10268-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

1X10^6 NIH/3T3 cells were stained with 0.2ug IkB alpha antibody (10268-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.