Name: IkB alpha Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 10268-1-AP
Rating: 4.00 (1 reviews)

Tested Applications

Applications:

WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee

IkB alpha Antibody WB HeLa cells 10268-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IkB alpha Antibody IHC human kidney tissue 10268-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

IkB alpha Antibody IF HeLa cells 10268-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 10268-1-AP(IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IkB alpha Antibody IP HeLa cells 10268-1-AP

IP Result of anti-IkB alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:1000) with HeLa cells lysate 4000ug.

IkB alpha Antibody FC HeLa cells 10268-1-AP

1X10^6 HeLa cells were stained with 0.2ug IkB alpha antibody (10268-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:

human, mouse, pig, A. sphaerocephala Krasch, broiler chick, CHICKEN, rat, swine

Positive WB detected in:

HeLa cells, A431 cells, HEK-293 cells, HL-60 cells, Jurkat cells, pig liver tissue, HepG2 cells, NIH/3T3 cells, mouse liver tissues

Positive IP detected in:

HeLa cells

Positive IHC detected in:

human kidney tissue, human prostate cancer tissue, human stomach tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HeLa cells

Positive FC detected in:

HeLa cells, NIH/3T3 cells

Recommended dilution:

WB : 1:1000-1:4000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:50-1:500

IF : 1:50-1:500

Sample-dependent, check data in Validation tab

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC.Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

IkB alpha fusion protein Ag0345

Full name:

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

Calculated molecular weight:

36 kDa

Observed molecular weight:

36 kDa

GenBank accession number:

BC002601

Gene ID (NCBI):

4792

Gene symbol

NFKBIA

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Background

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

IkB alpha Rabbit Polyclonal antibody

CHAO (Verified Customer), 01-30-2020

Applications

Western Blot,

No image submitted with review

Cell/Tissue Type

Mouse Heart

Lot Number

Weak bands for the heart lysates at basal state.

Showing 1- [6] of [119] Publications

Author:		Tang Siwei S
Pubmed ID:		23472066
Journal:		PLoS One
Title:		14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage.
Application:		WB

Species:		human

Author:		Zhang Liuping L
Pubmed ID:		23535871
Journal:		J Cancer Res Clin Oncol
Title:		MG132-mediated inhibition of the ubiquitin-proteasome pathway ameliorates cancer cachexia.
Application:		WB

Species:		mouse

Author:		Luo Jing J
Pubmed ID:		23450368
Journal:		Int J Oncol
Title:		The hepatitis B virus X protein downregulates NF-κB signaling pathways through decreasing the Notch signaling pathway in HBx-transformed L02 cells.
Application:		WB

Species:		human

Author:		Yanfei Li
Pubmed ID:		24583048
Journal:		Toxicol Lett
Title:		Chlorogenic acid prevents isoproterenol-induced hypertrophy in neonatal rat myocytes.
Application:		WB

Species:		rat

Author:		Kezhen Wang
Pubmed ID:		24807716
Journal:		J Virol
Title:		Herpes simplex virus type 1 protein kinase US3 hyperphosphorylates p65/RelA and dampens NF-κB activation.
Application:		WB

Species:		human

Author:		Xiujuan Shi
Pubmed ID:		25394920
Journal:		Int J Mol Med
Title:		Scutellarein inhibits cancer cell metastasis in vitro and attenuates the development of fibrosarcoma in vivo.
Application:

Species:		human

WB Figures

IkB alpha Antibody WB A431 cells 10268-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IkB alpha Antibody WB HEK-293 cells 10268-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IkB alpha Antibody WB HL-60 cells 10268-1-AP

HL-60 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP(IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IkB alpha Antibody WB Jurkat cells 10268-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10268-1-AP( IkB alpha antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

IkB alpha Antibody WB pig liver tissue 10268-1-AP

pig liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP( IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

IkB alpha Antibody IHC human prostate cancer tissue 10268-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

IkB alpha Antibody IHC human stomach tissue 10268-1-AP

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

IP Figures

IP Result of anti-IkB alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:1000) with HeLa cells lysate 4000ug.

IF Figures

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 10268-1-AP(IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

FC Figures

IkB alpha Antibody FC NIH/3T3 cells 10268-1-AP

1X10^6 NIH/3T3 cells were stained with 0.2ug IkB alpha antibody (10268-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

IkB alpha Antibody 119 Publications

Rabbit Polyclonal| Catalog number: 10268-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Customer Reviews

IkB alpha Rabbit Polyclonal antibody

Showing 1- [6] of [119] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

FC Figures

Product-specific protocols

Standard Protocols

IkB alpha Antibody 119 Publications

Rabbit Polyclonal| Catalog number: 10268-1-AP

-_-

Related Primary Antibodies

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Tested Applications

Product Information

Immunogen Information

Background

Customer Reviews

IkB alpha Rabbit Polyclonal antibody