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IkB alpha Antibody 73 Publications

Rabbit Polyclonal| Catalog number: 10268-1-AP

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Con: 24 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

IkB alpha Antibody WB Jurkat cells 10268-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10268-1-AP( IkB alpha antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

IkB alpha Antibody IHC human kidney tissue 10268-1-AP

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 10x lens).

IkB alpha Antibody IHC human kidney tissue 10268-1-AP

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP( IkB alpha antibody at dilution of 1:200 (under 40x lens).

IkB alpha Antibody IF HeLa cells 10268-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 10268-1-AP(IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IkB alpha Antibody IP HeLa cells 10268-1-AP

IP Result of anti-IkB alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:1000) with HeLa cells lysate 4000ug.

IkB alpha Antibody FC HeLa cells 10268-1-AP

1X10^6 HeLa cells were stained with 0.2ug IkB alpha antibody (10268-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, pig, A. sphaerocephala Krasch, rat
Positive WB detected in:
Jurkat cells, A431 cells, HEK-293 cells, HeLa cells, HL-60 cells, mouse liver tissue, NIH/3T3 cells, pig liver tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human kidney tissue, human prostate cancer tissue, human stomach tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells, NIH/3T3 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IkB alpha fusion protein Ag0345
Full name:
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight:
36 kDa
Observed molecular weight:
36 kDa
GenBank accession number:
BC002601
Gene ID (NCBI):
4792
Gene symbol
NFKBIA
Synonyms
I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Background

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.


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