IkB Alpha Polyclonal antibody
IkB Alpha Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (4)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
162
Cat no : 10268-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, HL-60 cells, Jurkat cells, pig liver tissue, HepG2 cells, NIH/3T3 cells, mouse liver tissues |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue, human stomach tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 154 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
Product Information
10268-1-AP targets IkB Alpha in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | A. sphaerocephala Krasch, CHICKEN, human, mouse, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IkB Alpha fusion protein Ag0345 |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC002601 |
| Gene symbol | NFKBIA |
| Gene ID (NCBI) | 4792 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| IHC protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| IF protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| IP protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| FC protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. | ||
Int Immunopharmacol Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice. | ||
Molecules Effects of Betaine on LPS-Stimulated Activation of Microglial M1/M2 Phenotypes by Suppressing TLR4/NF-κB Pathways in N9 Cells. | ||
J Hematol Oncol Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer. | ||
Biochem Pharmacol Betulinic acid suppresses breast cancer aerobic glycolysis via caveolin-1/NF-κB/c-Myc pathway. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH CHAO (Verified Customer) (01-30-2020) | Weak bands for the heart lysates at basal state.
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