CoraLite®647-conjugated IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite®647 Fluorescent Dye

Cat no : CL647-10268

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10268-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL647-10268 targets IkB Alpha in IF applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IkB Alpha fusion protein Ag0345
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
GenBank accession numberBC002601
Gene symbol NFKBIA
Gene ID (NCBI) 4792
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 665 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Protocols

Product Specific Protocols
IF protocol for CL647 IkB Alpha antibody CL647-10268Download protocol
Standard Protocols
Click here to view our Standard Protocols