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NKp46/NCR1 Recombinant monoclonal antibody (84416-5-RR)

NKp46/NCR1 Recombinant monoclonal antibody

NKp46/NCR1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84416-5-RR

Clone No.241811H7

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

241811H7, Activating receptor 1, Ar1, CD335, Kilr1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

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WB analysis of mouse splenocytes using 84416-5-RR
mouse splenocytes were subjected to SDS PAGE followed by western blot with 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat thymus using 84416-5-RR
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat spleen using 84416-5-RR
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat spleen using 84416-5-RR
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of rat spleen using 84416-5-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of rat spleen using 84416-5-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse spleen using 84416-5-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse spleen using 84416-5-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 84416-5-RR
Biolayer interferometry (BLl) kinetic assays of 84416-5-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.593 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse splenocytes
Positive IHC detected in	rat spleen tissue, rat thymus tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat spleen tissue, mouse spleen tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84416-5-RR targets NKp46/NCR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity	mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1469 Product name: Recombinant Rat NKp46/NCR1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 17-258 aa of NM_057199.1 Sequence: QRINTEKQTLPKPIIWAKPSIMVTKGNSVNIWCQGAQSASEYQLYFEGSFFALERPKSSRSMNKVKFFISQMTSHTAGIYTCFYQSGELWSESSNPLKLVVTGLYDTPTLWVHPGPEVTLGENVTFSCHLKTATSKFFLLKERESNHIQHKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLITGEVENTSLAPTDPVSSLDYWEFDLSTKESGLQKDSAFWDHTAQNLIR Predict reactive species
Full Name	natural cytotoxicity triggering receptor 1
Calculated Molecular Weight	37kDa
Observed Molecular Weight	50-60 kDa
GenBank Accession Number	NM_057199.1
Gene Symbol	NKp46/NCR1
Gene ID (NCBI)	117547
RRID	AB_3671945
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q9Z0H5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

Protocols

Product Specific Protocols
IF protocol for NKp46/NCR1 antibody 84416-5-RR	Download protocol
IHC protocol for NKp46/NCR1 antibody 84416-5-RR	Download protocol
WB protocol for NKp46/NCR1 antibody 84416-5-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	IHC	BMC Cancer Regulation of natural killer cell infiltration and tumor progression by CMTM6 in gastric cancer. Authors - Fangzhou Ye View Article
mouse	IF	Int J Genomics Identifying the Key Mitochondria-Related Genes in COPD by Integrating Machine Learning and Bioinformatics Analyses. Authors - Jiajia Qu View Article