CoraLite®594-conjugated NKp46/NCR1 Recombinant monoclonal antibody

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  IF Staining of rat spleen using CL594-84416-5

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CoraLite®594 NKp46/NCR1 antibody (CL594-84416-5, Clone: 241811H7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NKp46/NCR1 Uni-rAb^® Recombinant Antibody for IF-P

Cat No. CL594-84416-5

Clone No.241811H7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IF-P

Synonyms

Activating receptor 1, Ar1, CD335, Kilr1, Ly94

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Tested Applications

• Positive IF-P detected in: rat spleen tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-84416-5 targets NKp46/NCR1 in IF-P applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1469

  Product name: Recombinant Rat NKp46/NCR1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 17-258 aa of NM_057199.1

  Sequence: QRINTEKQTLPKPIIWAKPSIMVTKGNSVNIWCQGAQSASEYQLYFEGSFFALERPKSSRSMNKVKFFISQMTSHTAGIYTCFYQSGELWSESSNPLKLVVTGLYDTPTLWVHPGPEVTLGENVTFSCHLKTATSKFFLLKERESNHIQHKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLITGEVENTSLAPTDPVSSLDYWEFDLSTKESGLQKDSAFWDHTAQNLIR

• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 37kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_057199.1
• Gene Symbol: NKp46/NCR1
• Gene ID (NCBI): 117547
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Excitation Laser: Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Z0H5
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).