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  • KD/KO Validated

NLRX1 Antibody

NLRX1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 17215-1-AP

Synonyms

Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9



Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, MCF-7 cells, HepG2 cells, mouse heart tissue, mouse colon tissue, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human liver tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17215-1-AP targets NLRX1 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLRX1 fusion protein Ag11000
Full Name NLR family member X1
Calculated molecular weight 975aa,108 kDa
Observed molecular weight 100-110 kDa
GenBank accession number BC013199
Gene symbol NLRX1
Gene ID (NCBI) 79671
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NLRX1 antibody 17215-1-APDownload protocol
IHC protocol for NLRX1 antibody 17215-1-APDownload protocol
IF protocol for NLRX1 antibody 17215-1-APDownload protocol
IP protocol for NLRX1 antibody 17215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Innate Immun

NLRX1 does not inhibit MAVS-dependent antiviral signalling.

Authors - Fraser Soares
  • KO Validated
mouseWB,IF

Toxicol Appl Pharmacol

Activation of NLRX1-mediated autophagy accelerates the ototoxic potential of cisplatin in auditory cells.

Authors - Haiyan Yin
mouseWB

Mol Cell Biochem

The mitochondrial Nod-like receptor NLRX1 modifies apoptosis through SARM1.

Authors - Samuel A Killackey
humanWB,IF

Virus Res

Nucleotide-binding oligomerization domain-like receptor X1 restricts porcine reproductive and respiratory syndrome virus-2 replication by interacting with viral Nsp9.

Authors - Huiyuan Jing
  • KD Validated
mouseWB, IF

Mol Brain

Nlrx1 regulates neuronal cell death.

Authors - Emilie Imbeault
mouseWB

J Biol Chem

The mitochondrial protein NLRX1 controls the balance between extrinsic and intrinsic apoptosis.

Authors - Fraser Soares
  • KO Validated