Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, LNCaP cells, MCF-7 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11451-1-AP targets NQO1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, rabbit, chicken, hamster, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2009 Product name: Recombinant human NQO1 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-274 aa of BC007659 Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK Predict reactive species |
| Full Name | NAD(P)H dehydrogenase, quinone 1 |
| Calculated Molecular Weight | 274 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC007659 |
| Gene Symbol | NQO1 |
| Gene ID (NCBI) | 1728 |
| RRID | AB_2298729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NQO1 antibody 11451-1-AP | Download protocol |
| IF protocol for NQO1 antibody 11451-1-AP | Download protocol |
| IHC protocol for NQO1 antibody 11451-1-AP | Download protocol |
| IP protocol for NQO1 antibody 11451-1-AP | Download protocol |
| WB protocol for NQO1 antibody 11451-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NQO1 polyclonal antibody (Cat# 11451-1-AP) has accumulated 549 citations in high-impact journals including Nature, Science, Cell Metabolism, Nature Communications, Redox Biology, Molecular Cell, Free Radical Biology and Medicine, and Phytomedicine. Associated research fields encompass ferroptosis, oxidative stress, Nrf2/Keap1 signaling, cancer biology, neurodegeneration, hepatology, nephrology, cardiovascular disease, inflammation, autophagy, medicinal chemistry, and nutritional science.
| Species | Application | Title |
|---|---|---|
Nature The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.
| ||
Nanomicro Lett A Rational Design of Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal Burns | ||
Cell Metab A clinical-stage Nrf2 activator suppresses osteoclast differentiation via the iron-ornithine axis | ||
Adv Mater Functionalized Nanozyme Microcapsules Targeting Deafness Prevention via Mitochondrial Homeostasis Remodeling | ||
Adv Mater A Supramolecular Nanoengine Generates Nanomechanical Force on Demand for Precise Cytosolic Delivery of Anti-miRNAs and Synergistic TNBC Therapy |
