|Positive WB detected in||HEK-293 cells, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24858-1-AP is NRBF2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NRBF2 fusion protein Ag21403|
|Full Name||nuclear receptor binding factor 2|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||35-37 kDa|
|GenBank accession number||BC001345|
|Gene ID (NCBI)||29982|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.
|Product Specific Protocols|
|WB protocol for NRBF2 antibody 24858-1-AP||Download protocol|
|IHC protocol for NRBF2 antibody 24858-1-AP||Download protocol|
|IF protocol for NRBF2 antibody 24858-1-AP||Download protocol|
|IP protocol for NRBF2 antibody 24858-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.
NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.
Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.