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  • KD/KO Validated

NRBF2 Polyclonal antibody

NRBF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 24858-1-AP


Comodulator of PPAR and RXR, COPR, COPR1, COPR2, NRBF 2, NRBF2

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24858-1-AP is NRBF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRBF2 fusion protein Ag21403
Full Name nuclear receptor binding factor 2
Calculated molecular weight 32 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC001345
Gene symbol NRBF2
Gene ID (NCBI) 29982
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.


Product Specific Protocols
WB protocol for NRBF2 antibody 24858-1-APDownload protocol
IHC protocol for NRBF2 antibody 24858-1-APDownload protocol
IF protocol for NRBF2 antibody 24858-1-APDownload protocol
IP protocol for NRBF2 antibody 24858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Neuroinflammation

Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.

Authors - Hanhai Zeng
  • KO Validated


NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.

Authors - Weitao Shen
  • KD Validated

Lab Invest

Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.

Authors - Xiaosen Ouyang