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  • KD/KO Validated

NRBF2 Polyclonal antibody

NRBF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24858-1-AP

Synonyms

Comodulator of PPAR and RXR, COPR, COPR1, COPR2, NRBF 2, NRBF2



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24858-1-AP is NRBF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRBF2 fusion protein Ag21403
Full Name nuclear receptor binding factor 2
Calculated molecular weight 32 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC001345
Gene symbol NRBF2
Gene ID (NCBI) 29982
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for NRBF2 antibody 24858-1-APDownload protocol
IHC protocol for NRBF2 antibody 24858-1-APDownload protocol
IF protocol for NRBF2 antibody 24858-1-APDownload protocol
IP protocol for NRBF2 antibody 24858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC,IF

J Neuroinflammation

Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.

Authors - Hanhai Zeng
  • KO Validated
human,mouseWB,IHC,IF,CoIP

iScience

NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.

Authors - Weitao Shen
  • KD Validated
mouseWB

Lab Invest

Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.

Authors - Xiaosen Ouyang