NUB1 Polyclonal antibody

NUB1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14343-1-AP

Synonyms

BS4, NEDD8 ultimate buster 1, NUB1, NUB1L, NYREN18



Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells, K-562 cells, mouse kidney tissue, Raji cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14343-1-AP targets NUB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUB1 fusion protein Ag5668
Full Name negative regulator of ubiquitin-like proteins 1
Calculated molecular weight 71 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC046354
Gene symbol NUB1
Gene ID (NCBI) 51667
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.

Protocols

Product Specific Protocols
WB protocol for NUB1 antibody 14343-1-APDownload protocol
IP protocol for NUB1 antibody 14343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Neuropathology

Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.

Authors - Fumiaki Mori
mouseWB,IF

Biochem Biophys Res Commun

The role of NUB1 in α-synuclein degradation in Lewy body disease model mice.

Authors - Kunikazu Tanji