OFD1 Polyclonal Antibody for IHC, WB, ELISA
Cat no : 22851-1-AP
71 7A, CXorf5, OFD1, oral facial digital syndrome 1, Protein 71 7A, SGBS2
|Positive WB detected in||HeLa cells, SH-SY5Y cells|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The 22851-1-AP immunogen is Fusion Protein OFD1 protein expressed in E. coli.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OFD1 fusion protein Ag18898|
|Full Name||oral-facial-digital syndrome 1|
|Calculated molecular weight||1012aa,117 kDa|
|Observed molecular weight||43 kda|
|GenBank accession number||BC096344|
|Gene ID (NCBI)||8481|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
OFD1,also named as CXorf5 and 71-7A, belongs to the OFD1 family. It has been implicated in several developmental syndromes, including a male-lethal X-linked dominant condition,Oral-Facial-Digital type 1 (OFD1) syndrome, X-linked recessiveSimpson-Golabi-Behmel syndrome type 2 (SGBS2) and Joubert syndrome and related disorders (JSRDs). It is a component of the centrioles controlling mother and daughter centrioles length. It is recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. OFD1 plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. This antibody recognize all the isoforms of OFD1. The 43 kDa is isoform 2. The ~80 kDa band is unknown.
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