OFD1 Antibody

OFD1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22851-1-AP

Synonyms

71 7A, CXorf5, OFD1, oral facial digital syndrome 1, Protein 71 7A, SGBS2



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 22851-1-AP immunogen is Fusion Protein OFD1 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OFD1 fusion protein Ag18898
Full Name oral-facial-digital syndrome 1
Calculated molecular weight 1012aa,117 kDa
Observed molecular weight 43 kda
GenBank accession number BC096344
Gene symbol OFD1
Gene ID (NCBI) 8481
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OFD1,also named as CXorf5 and 71-7A, belongs to the OFD1 family. It has been implicated in several developmental syndromes, including a male-lethal X-linked dominant condition,Oral-Facial-Digital type 1 (OFD1) syndrome, X-linked recessiveSimpson-Golabi-Behmel syndrome type 2 (SGBS2) and Joubert syndrome and related disorders (JSRDs). It is a component of the centrioles controlling mother and daughter centrioles length. It is recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. OFD1 plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. This antibody recognize all the isoforms of OFD1. The 43 kDa is isoform 2. The ~80 kDa band is unknown.

Protocols

Product Specific Protocols
WB protocol for OFD1 antibody 22851-1-APDownload protocol
IHC protocol for OFD1 antibody 22851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Death Dis

Primary cilia mediate mitochondrial stress responses to promote dopamine neuron survival in a Parkinson's disease model.

Authors - Ji-Eun Bae
ratWB

Biochem Biophys Res Commun

BIX-01294-induced autophagy regulates elongation of primary cilia.

Authors - Ji Hyun Shin
humanIF

PLoS Biol

Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.

Authors - Özge Z Aydin
humanIF

Nat Commun

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.

Authors - Dragomir B Krastev