OLIG2 Polyclonal antibody

OLIG2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25754-1-AP

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17



Tested Applications

Positive WB detected inrat brain tissue, C6 cells
Positive IHC detected inhuman gliomas tissue, mouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25754-1-AP is OLIG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLIG2 fusion protein Ag22642
Full Name oligodendrocyte lineage transcription factor 2
Calculated molecular weight 32 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC047511
Gene symbol OLIG2
Gene ID (NCBI) 10215
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.

Protocols

Product Specific Protocols
WB protocol for OLIG2 antibody 25754-1-APDownload protocol
IHC protocol for OLIG2 antibody 25754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols