Biotin-conjugated OPTN Monoclonal antibody

OPTN Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Biotin Fluorescent Dye

Cat no : Biotin-60293

Synonyms

FIP 2, optineurin, OPTN



Tested Applications

Positive WB detected inhuman brain
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

Biotin-60293 targets OPTN in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OPTN fusion protein Ag1272
Full Name optineurin
Calculated molecular weight 66 kDa
GenBank accession numberBC013876
Gene symbol OPTN
Gene ID (NCBI) 10133
Conjugate Biotin Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS(10mM), with 50% glycerol, without sodium azide.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. This antibody is conjugated with Biotin.

Protocols

Product Specific Protocols
IHC protocol for Biotin-conjugated OPTN antibody Biotin-60293Download protocol
Standard Protocols
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