Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, pig skeletal muscle tissue |
| Positive IHC detected in | human pancreas cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60293-1-Ig targets OPTN/Optineurin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1272 Product name: Recombinant human OPTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-577 aa of BC013876 Sequence: VSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII Predict reactive species |
| Full Name | optineurin |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 70-74 kDa |
| GenBank Accession Number | BC013876 |
| Gene Symbol | OPTN |
| Gene ID (NCBI) | 10133 |
| RRID | AB_2881408 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96CV9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OPTN/Optineurin antibody 60293-1-Ig | Download protocol |
| IHC protocol for OPTN/Optineurin antibody 60293-1-Ig | Download protocol |
| WB protocol for OPTN/Optineurin antibody 60293-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OPTN/Optineurin Monoclonal antibody (Cat# 60293-1-Ig) has 3 citations. They appear in Autophagy (ferroptosis and copper-dependent autophagic degradation of GPX4), Developmental Cell (PDGF signaling, mitophagy, and N6-methyladenosine in glioblastoma stem cells), and F1000Research (antibody validation for Optineurin in WB, IP, and IF). Research fields span ferroptosis, autophagy, neuro-oncology, RNA modification, and antibody benchmarking.
| Species | Application | Title |
|---|---|---|
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.
| ||
F1000Res A guide to selecting high-performing antibodies for Optineurin (UniProt ID: Q96CV9) for use in western blot, immunoprecipitation, and immunofluorescence.
|
Reviews
What customers say
The antibody received a 5-star rating for its use in Western Blot on iPSCs at a 1:4000 dilution. The reviewer included an image demonstrating clear and specific band detection, with no written comments. Overall, the single review reflects strong performance and satisfaction with the product's sensitivity and specificity in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Malika (Verified Customer) (10-02-2025) |
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