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  • KD/KO Validated

PADI4 Polyclonal antibody

PADI4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17373-1-AP

Synonyms

HL 60 PAD, PAD, PAD4, PADI4, PADI5, PDI4, PDI5, Peptidylarginine deiminase IV



Tested Applications

Positive WB detected inHEK-293 cells, Purified protein, HepG2 cells, C6 cells, RAW 264.7 cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17373-1-AP is PADI4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PADI4 fusion protein Ag11068
Full Name peptidyl arginine deiminase, type IV
Calculated molecular weight 663 aa, 74 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC025718
Gene symbol PADI4
Gene ID (NCBI) 23569
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PADI4, also named as PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues.(PMID: 19183436)

Protocols

Product Specific Protocols
WB protocol for PADI4 antibody 17373-1-APDownload protocol
IHC protocol for PADI4 antibody 17373-1-APDownload protocol
IF protocol for PADI4 antibody 17373-1-APDownload protocol
IP protocol for PADI4 antibody 17373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Front Immunol

Thioredoxin Modulates Protein Arginine Deiminase 4 (PAD4)-Catalyzed Citrullination.

Authors - Mitesh Nagar
mouseWB

Sci Rep

Cold-inducible RNA-binding Protein Induces Neutrophil Extracellular Traps in the Lungs during Sepsis.

Authors - Yasumasa Ode
mouseWB,IF

Invest Ophthalmol Vis Sci

Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4).

Authors - T J Hollingsworth
  • KO Validated
mouseIF

Cells

Innate and Autoimmunity in the Pathogenesis of Inherited Retinal Dystrophy.

Authors - T J Hollingsworth
mouseWB

RMD Open

IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.

Authors - Ayano Yahagi

BMC Musculoskelet Disord

Decreased severity of experimental autoimmune arthritis in peptidylarginine deiminase type 4 knockout mice.

Authors - Akari Suzuki