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KD/KO Validated

PAK1 Polyclonal antibody

Name: PAK1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 21401-1-AP

PAK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21401-1-AP

Synonyms

Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha

Validation Data Gallery

WB analysis using 21401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 21401-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 21401-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of K-562 using 21401-1-AP
IP Result of anti-PAK1 (IP:21401-1-AP, 4ug; Detection:21401-1-AP 1:1000) with K-562 cells lysate 3200ug.
IF Staining of C6 using 21401-1-AP
Immunofluorescent analysis of C6 cells using 21401-1-AP (PAK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 21401-1-AP

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Tested Applications

Positive WB detected in	C6 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, HeLa cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse brain tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	C6 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

The immunogen of 21401-1-AP is PAK1 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PAK1 fusion protein Ag16102
Full Name	p21 protein (Cdc42/Rac)-activated kinase 1
Calculated molecular weight	553 aa, 62 kDa
Observed molecular weight	61-65 kDa
GenBank accession number	BC109299
Gene symbol	PAK1
Gene ID (NCBI)	5058
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PAK1 antibody 21401-1-AP	Download protocol
IHC protocol for PAK1 antibody 21401-1-AP	Download protocol
IF protocol for PAK1 antibody 21401-1-AP	Download protocol
IP protocol for PAK1 antibody 21401-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun P-21-activated kinase 1 contributes to tumor angiogenesis upon photodynamic therapy via the HIF-1α/VEGF pathway. Authors - Shuai Jiang KD Validated View Article
rat	CoIP	Brain Res Bull Adenoviral Vector-Induced Silencing of RGMa Attenuates Blood-Brain Barrier Dysfunction in a Rat Model of MCAO/Reperfusion. Authors - Min Li View Article
human	WB	Cell Signal Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis. Authors - Fan Zhang View Article
human	WB	Gynecol Oncol NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway. Authors - Pei Xia View Article
mouse	WB	Sci Rep Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module. Authors - Dongli Liang View Article
mouse	IF,WB	Theranostics Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development. Authors - Min Zhang View Article

PAK1 Polyclonal antibody

PAK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 21401-1-AP

IHC staining of mouse brain using 21401-1-AP

IHC staining of mouse brain using 21401-1-AP

IP experiment of K-562 using 21401-1-AP

IF Staining of C6 using 21401-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

P-21-activated kinase 1 contributes to tumor angiogenesis upon photodynamic therapy via the HIF-1α/VEGF pathway.

Adenoviral Vector-Induced Silencing of RGMa Attenuates Blood-Brain Barrier Dysfunction in a Rat Model of MCAO/Reperfusion.

Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.

NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway.

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.