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  • KD/KO Validated

PAK1 Polyclonal antibody

PAK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21401-1-AP

Synonyms

Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha



Tested Applications

Positive WB detected inC6 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, HeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21401-1-AP is PAK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAK1 fusion protein Ag16102
Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Calculated molecular weight 553 aa, 62 kDa
Observed molecular weight 61-65 kDa
GenBank accession numberBC109299
Gene symbol PAK1
Gene ID (NCBI) 5058
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PAK1 antibody 21401-1-APDownload protocol
IHC protocol for PAK1 antibody 21401-1-APDownload protocol
IF protocol for PAK1 antibody 21401-1-APDownload protocol
IP protocol for PAK1 antibody 21401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

P-21-activated kinase 1 contributes to tumor angiogenesis upon photodynamic therapy via the HIF-1α/VEGF pathway.

Authors - Shuai Jiang
  • KD Validated
ratCoIP

Brain Res Bull

Adenoviral Vector-Induced Silencing of RGMa Attenuates Blood-Brain Barrier Dysfunction in a Rat Model of MCAO/Reperfusion.

Authors - Min Li
humanWB

Cell Signal

Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.

Authors - Fan Zhang
humanWB

Gynecol Oncol

NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway.

Authors - Pei Xia
mouseWB

Sci Rep

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Authors - Dongli Liang
mouseIF,WB

Theranostics

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Authors - Min Zhang