Various lysates were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

C6 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP(PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 21401-1-AP(PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 21401-1-AP(PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP(PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP(PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP(PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human breast cancer slide using 21401-1-AP( PAK1 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded human breast cancer slide using 21401-1-AP( PAK1 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-PAK1 (IP:21401-1-AP, 4ug; Detection:21401-1-AP 1:1000) with K-562 cells lysate 3200ug.

Immunofluorescent analysis of C6 cells using 21401-1-AP(PAK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)