PALB2 Polyclonal antibody

PALB2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14340-1-AP

Synonyms

FANCN, PALB2, partner and localizer of BRCA2



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14340-1-AP targets PALB2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PALB2 fusion protein Ag5654
Full Name partner and localizer of BRCA2
Calculated molecular weight 131 kDa
Observed molecular weight 130 kDa; 80-90 kDa; 250-260 kDa
GenBank accession numberBC044254
Gene symbol PALB2
Gene ID (NCBI) 79728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PALB2 can interact with BRCA1 and BRCA2 and form the BRCA1-PALB2-BRCA2 complex which is critical for homologous recombination and DNA damage response. PALB2 is an essential partner of BRCA2 that promotes the localization and stability of BRCA2. The predicted MW of PALB2 is approximately 130 kDa, while the truncated PALB2 protein arround 80-90 kDa can also be detected in SDS-PAGE similar to papers. (PubMed:16793542, 24141787, 24153426). There's the dimer form with WM 250-260 kDa (refer to OMID: 30289697).

Protocols

Product Specific Protocols
WB protocol for PALB2 antibody 14340-1-APDownload protocol
IHC protocol for PALB2 antibody 14340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region.

Authors - Zhihao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (06-04-2021)

Do not see the band at correct molecular weight

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Fallopian tube epithelial cells
PALB2 Antibody Western Blot validation (1:500 dilution) in Fallopian tube epithelial cells (Cat no:14340-1-AP)