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PAMR1 Polyclonal antibody

PAMR1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 55310-1-AP

Synonyms

DKFZP586H2123, FP938, Inactive serine protease PAMR1, PAMR1, RAMP, UNQ699/PRO1344

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PAMR1 Polyclonal antibody (55310-1-AP)

    WB analysis of mouse skeletal muscle using 55310-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55310-1-AP (PAMR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PAMR1 Polyclonal antibody (55310-1-AP)

    IHC staining of human skeletal muscle using 55310-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55310-1-AP (PAMR1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PAMR1 Polyclonal antibody (55310-1-AP)

    IHC staining of human skeletal muscle using 55310-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55310-1-AP (PAMR1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55310-1-AP targets PAMR1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name regeneration associated muscle protease
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 78-82 kDa, 67-70 kDa
GenBank Accession NumberNM_015430
Gene Symbol DKFZP586H2123
Gene ID (NCBI) 25891
RRIDAB_11232034
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAMR1,also named as RAMP, belongs to the peptidase S1 family. It may play a role in regeneration of skeletal muscle. This antibody is specific to PAMR1.

Protocols

Product Specific Protocols
WB protocol for PAMR1 antibody 55310-1-APDownload protocol
IHC protocol for PAMR1 antibody 55310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Elife

The subiculum is a patchwork of discrete subregions.

Authors - Mark S Cembrowski
View Article
humanWB, IHC

Front Oncol

High Expression of PAMR1 Predicts Favorable Prognosis and Inhibits Proliferation, Invasion, and Migration in Cervical Cancer.

Authors - Rui Yang
View Article