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PCNA Polyclonal antibody

PCNA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 10205-2-AP

Synonyms

PCNA


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  • Western Blot (WB) analysis of HEK293 cells using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of HEK293 cells using 10205-2-AP

    WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells.

  • Western Blot (WB) analysis of various lysates using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis using 10205-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis using 10205-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of rat testis using 10205-2-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of mouse testis using 10205-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of Raji using 10205-2-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of Jurkat using 10205-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of mouse liver using 10205-2-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of A431 using 10205-2-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of NIH/3T3 using 10205-2-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of HepG2 using 10205-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using PCNA Polyclonal antibody (10205-2-AP)

    WB analysis of rat liver using 10205-2-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PCNA Polyclonal antibody (10205-2-AP)

    IP experiment of MCF-7 using 10205-2-AP

    IP Result of anti-PCNA (IP:10205-2-AP, 3ug; Detection:10205-2-AP 1:1500) with MCF-7 cells lysate 2500ug.

  • Immunoprecipitation (IP) experiment using PCNA Polyclonal antibody (10205-2-AP)

    IP experiment using 10205-2-AP

    IP result of anti-PCNA (10205-2-AP for IP and Detection) with MCF7 cell.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human stomach cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10205-2-AP (PCNA antibody) at dilution of 1:500 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human liver cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human liver cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human stomach cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10205-2-AP (PCNA antibody) at dilution of 1:500 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of mouse heart using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of mouse liver using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of mouse liver using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human colon cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human malignant melanoma using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human malignant melanoma using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human liver cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human liver cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human liver cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human breast cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IHC staining of human breast cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCNA Polyclonal antibody (10205-2-AP)

    IF Staining of HepG2 using 10205-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCNA antibody (10205-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL94-phalloidin (red), DAPI (Blue).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCNA Polyclonal antibody (10205-2-AP)

    IF Staining of HepG2 using 10205-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCNA Polyclonal antibody (10205-2-AP)

    IF Staining of MCF-7 using 10205-2-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCNA antibody (10205-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PCNA Polyclonal antibody (10205-2-AP)

    IF Staining of mouse testis using 10205-2-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PCNA antibody (10205-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PCNA Polyclonal antibody (10205-2-AP)

    IF Staining of Neuro-2a using 10205-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using PCNA Polyclonal antibody (10205-2-AP)

    IF Staining of human breast cancer using 10205-2-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10205-2-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using PCNA Polyclonal antibody (10205-2-AP)

    FC experiment of Jurkat using 10205-2-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PCNA (10205-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

"PCNA Antibodies" Comparison

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Tested Applications

Positive WB detected inA431 cells, NIH/3T3 cells, mouse testis tissue, Raji cells, Jurkat cells, mouse liver tissue, rat liver tissue, rat testis tissue, HEK293 cells, HepG2 cells, HeLa cells, MCF-7 cells, PC-12 cells, C2C12 cells, mouse spleen tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human stomach cancer tissue, human malignant melanoma tissue, human breast cancer tissue, human colon cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human breast cancer tissue, MCF-7 cells, mouse testis tissue, Neuro-2a cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

10205-2-AP targets PCNA in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Alligator, bovine, chicken, ducks, fish, hamster, medaka embryos, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCNA fusion protein Ag0277
Full Name proliferating cell nuclear antigen
Calculated molecular weight 29 kDa/31 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC000491
Gene symbol PCNA
Gene ID (NCBI) 5111
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Protocols

Product Specific Protocols
WB protocol for PCNA antibody 10205-2-APDownload protocol
IHC protocol for PCNA antibody 10205-2-APDownload protocol
IF protocol for PCNA antibody 10205-2-APDownload protocol
IP protocol for PCNA antibody 10205-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomaterials

PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy

Authors - Lu Wang
mouseWB

Hepatology

Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.

Authors - Yu Sun
humanWB, IHC

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui
mouseIHC

Dev Cell

Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development

Authors - Xue-Lian Sun
humanWB

Nat Commun

Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway.

Authors - Jingbo Dai
humanIF

Dev Cell

Cdk1 Activates Pre-mitotic Nuclear Envelope Dynein Recruitment and Apical Nuclear Migration in Neural Stem Cells.

Authors - Alexandre D Baffet

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Nice antibody which worked well on PC3 cells fixed with 4% PFA. Antibody used at 1:50

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: PC3 cells
PCNA Antibody Immunofluorescence validation (1:50 dilution) in PC3 cells (Cat no:10205-2-AP)