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PCNA Polyclonal antibody
PCNA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (8)
WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA
Cat no : 10205-2-AP
"PCNA Antibodies" Comparison
View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||A431 cells, NIH/3T3 cells, mouse testis tissue, Raji cells, Jurkat cells, mouse liver tissue, rat liver tissue, rat testis tissue, HEK293 cells, HepG2 cells, HeLa cells, MCF-7 cells, PC-12 cells, C2C12 cells, mouse spleen tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human liver cancer tissue, human stomach cancer tissue, human malignant melanoma tissue, human breast cancer tissue, human colon cancer tissue, mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, human breast cancer tissue, MCF-7 cells, mouse testis tissue, Neuro-2a cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 527 publications below|
|IHC||See 183 publications below|
|IF||See 59 publications below|
|CoIP||See 1 publications below|
10205-2-AP targets PCNA in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Alligator, bovine, chicken, ducks, fish, hamster, medaka embryos, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCNA fusion protein Ag0277|
|Full Name||proliferating cell nuclear antigen|
|Calculated molecular weight||29 kDa/31 kDa|
|Observed molecular weight||36-38 kDa|
|GenBank accession number||BC000491|
|Gene ID (NCBI)||5111|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
|Product Specific Protocols|
|WB protocol for PCNA antibody 10205-2-AP||Download protocol|
|IHC protocol for PCNA antibody 10205-2-AP||Download protocol|
|IF protocol for PCNA antibody 10205-2-AP||Download protocol|
|IP protocol for PCNA antibody 10205-2-AP||Download protocol|
|Click here to view our Standard Protocols|
PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy
Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.
J Pineal Res
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development
Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway.
Cdk1 Activates Pre-mitotic Nuclear Envelope Dynein Recruitment and Apical Nuclear Migration in Neural Stem Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Nice antibody which worked well on PC3 cells fixed with 4% PFA. Antibody used at 1:50