PCNA Antibody 86 Publications

Rabbit Polyclonal| Catalog number: 10205-2-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 38 μg/150 μl

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Species specificity:
human, mouse, rat, zebrafish

Positive WB detected in:
MCF7 cells, HEK293 cells

Positive IP detected in:
MCF-7 cells

Positive IHC detected in:
human stomach cancer tissue,human breast cancer tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HEK-293T cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
IHC : 1:200-1:1000
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
proliferating cell nuclear antigen

Calculated molecular weight:
29 kDa/31 kDa

Observed molecular weight:
36-38 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PCNA

Synonyms
PCNA
Background

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).


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