• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDE4C Polyclonal antibody

PDE4C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21754-1-AP

Synonyms

DPDE1, PDE21, PDE4C

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using PDE4C Polyclonal antibody (21754-1-AP)

    WB analysis of HEK-293 using 21754-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21754-1-AP (PDE4C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using PDE4C Polyclonal antibody (21754-1-AP)

    WB analysis of mouse lung using 21754-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21754-1-AP (PDE4C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

21754-1-AP targets PDE4C in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE4C fusion protein Ag16130
Full Name phosphodiesterase 4C, cAMP-specific (phosphodiesterase E1 dunce homolog, Drosophila)
Calculated Molecular Weight 712 aa, 80 kDa
Observed Molecular Weight~70 kDa
GenBank Accession NumberBC109067
Gene Symbol PDE4C
Gene ID (NCBI) 5143
RRIDAB_10860265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE4C(cAMP-specific 3,5-cyclic phosphodiesterase 4C) is also named as DPDE1 and belongs to the PDE4 subfamily, which play a key role in lung inflammation and hyperresponsiveness. PDE4C promotes the hydrolysis of cAMP, and PKD2, functioning as a Ca2+ entry channel, may mediate local accumulation of Ca2+ that inhibits the activity of Ca2+-sensitive ADCY5(PMID:21670265). The three isoforms(84,75, and 64 kDa) of PDE4C have been detected in cytosolic, microsomal, and nuclear (PMID:21784969).

Protocols

Product Specific Protocols
WB protocol for PDE4C antibody 21754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

JCI Insight

Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth

Authors - Mi-Hyeon Jeong
View Article
mouseWB

Mol Metab

Inhibition of prolyl hydroxylases increases hepatic insulin and decreases glucagon sensitivity by an HIF-2α-dependent mechanism.

Authors - Matthew Riopel
View Article
mouseWB

Biochem Pharmacol

DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.

Authors - Heng Li
View Article
mouseWB

Am J Physiol Renal Physiol

Inhibition of PDE4/PDE4B improves renal function and ameliorates inflammation in cisplatin-induced acute kidney injury.

Authors - Man Xu
View Article