Tested Applications
| Positive WB detected in | A375 cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15967-1-AP targets ERp57/ERp60 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8741 Product name: Recombinant human PDIA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 161-505 aa of BC014433 Sequence: DDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL Predict reactive species |
| Full Name | protein disulfide isomerase family A, member 3 |
| Calculated Molecular Weight | 505 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC014433 |
| Gene Symbol | PDIA3 |
| Gene ID (NCBI) | 2923 |
| RRID | AB_2236784 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30101 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| IHC protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| IP protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| WB protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PDIA3 antibody (Cat# 15967-1-AP) has 54 total citations published in journals including Cell, Nature Communications, Nature Cell Biology, EMBO Journal, Oncogene, Frontiers in Immunology, Molecular Metabolism, and Oncotarget. Research fields span endoplasmic reticulum stress and protein folding, cancer biology (ovarian, lung, prostate, esophageal, cervical, glioma), metabolic disorders (obesity, glucose homeostasis), bone biology, reproductive biology, cardiovascular disease, virology, and immune signaling.
| Species | Application | Title |
|---|---|---|
Cell Metab PDIA3 defines a novel subset of adipose macrophages to exacerbate the development of obesity and metabolic disorders | ||
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Reviews
What customers say
One customer rated this antibody 5 stars, reporting that among four PDIA3/ERp57 antibodies they tested from different companies, this one delivered the cleanest and strongest Western blot signal on normal rat kidney cells at a 1:1000 dilution. They also noted the diluted antibody was reusable across multiple blots and expressed intent to continue using it going forward.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Prasanna (Verified Customer) (05-07-2018) | Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.
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