|Positive WB detected in||HEK-293 cells, MCF-7 cells, K-562 cells, mouse pancreas tissue, HepG2 cells|
|Positive IHC detected in||human pancreas tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
20582-1-AP targets PERK/EIF2AK3 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bovine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||eukaryotic translation initiation factor 2-alpha kinase 3|
|Calculated molecular weight||125 kDa|
|Observed molecular weight||140 kDa|
|GenBank accession number||NM_004836|
|Gene ID (NCBI)||9451|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.
Br J Pharmacol
Betulinic acid alleviates endoplasmic reticulum stress-mediated NAFLD through activation of FXR in mice.
CNS Neurosci Ther
Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.
Br J Pharmacol
Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.
Quantitative proteomic analysis reveals unfolded protein response involved in severe fever with thrombocytopenia syndrome virus infection.
Int J Mol Med
Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Maria (Verified Customer) (02-10-2021)
PERK in human primary fibroblasts. 12% TGX gel. Blocking 1h RT 3% BSA in PBST (0.1% Tween). Primary incubation 16h 4ºC 1:1000. Secondary 1:5000 Goat anti-rabbit HRP 1h RT.
Aeid (Verified Customer) (11-12-2018)
Works fine for HEK293 but not for MEF or INS1 cells