PI16 Polyclonal antibody

PI16 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12267-1-AP

Synonyms

CRISP 9, CRISP9, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328



Tested Applications

Positive WB detected inSKOV-3 cells, A2780 cells, COLO 320 cells, A549 cells
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12267-1-AP targets PI16 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI16 fusion protein Ag2925
Full Name peptidase inhibitor 16
Calculated Molecular Weight 463 aa, 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC022399
Gene Symbol PI16
Gene ID (NCBI) 221476
RRIDAB_10697651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PI16 antibody 12267-1-APDownload protocol
IHC protocol for PI16 antibody 12267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIHC,WB,CoIP

Cell Mol Biol Lett

Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO

Authors - Xiangqin Kuang
  • KD Validated