Filter: AllWBIHC

WB analysis using 12267-1-AP (same clone as 12267-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
WB analysis using 12267-1-AP (same clone as 12267-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 12267-1-AP (same clone as 12267-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
WB analysis of A2780 using 12267-1-AP (same clone as 12267-1-PBS)
A2780 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 12267-1-AP (same clone as 12267-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 12267-1-AP (same clone as 12267-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PI16 Polyclonal antibody, PBS Only

PI16 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12267-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12267-1-PBS targets PI16 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2925 Product name: Recombinant human PI16 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC022399 Sequence: MHGSCSFLMLLLPLLLLLVATTGPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPSFRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSMATEAPPCVTTEVPSILAAHSLPSL Predict reactive species
Full Name	peptidase inhibitor 16
Calculated Molecular Weight	463 aa, 49 kDa
Observed Molecular Weight	49 kDa
GenBank Accession Number	BC022399
Gene Symbol	PI16
Gene ID (NCBI)	221476
RRID	AB_10697651
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6UXB8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).