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PKM Polyclonal antibody

PKM Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10078-2-AP

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK



Tested Applications

Positive WB detected inHeLa cells, multi-cells/tissue, HepG2 cells, SH-SY5Y cells, Jurkat cells, MCF-7 cells, SKOV-3 cells, NIH/3T3 cells, HEK-293 cells, Raji cells, mouse brain tissue, mouse muscle tissue, mouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

10078-2-AP targets PKM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKM fusion protein Ag0110
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC000481
Gene symbol PKM
Gene ID (NCBI) 5315
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.

Protocols

Product Specific Protocols
WB protocol for PKM antibody 10078-2-APDownload protocol
IHC protocol for PKM antibody 10078-2-APDownload protocol
IF protocol for PKM antibody 10078-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Pain Res

Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points.

Authors - Li-Hui Li
pigWB

Br J Pharmacol

PMID: 30515768

Authors - Xiaoying Lian
humanWB

Onco Targets Ther

WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.

Authors - Jingjing Dai

Analyst

An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.

Authors - Yu Yanyan Y
mouseWB

Am J Physiol Endocrinol Metab

PMID: 31211620

Authors - Yang Yang
pigWB

Sci Rep

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Authors - Liangliang Fu