PLCH1 Polyclonal antibody

PLCH1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19143-1-AP

Synonyms

KIAA1069, Phospholipase C eta 1, Phospholipase C like protein 3, phospholipase C, eta 1, PLC eta 1, PLC L3, PLCeta1, PLCH1, PLCL3



Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 19143-1-AP is PLCH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCH1 fusion protein Ag13607
Full Name phospholipase C, eta 1
Calculated Molecular Weight 1655 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC113950
Gene Symbol PLCH1
Gene ID (NCBI) 23007
RRIDAB_10643386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PLCH1 antibody 19143-1-APDownload protocol
IHC protocol for PLCH1 antibody 19143-1-APDownload protocol
IF protocol for PLCH1 antibody 19143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics

Authors - Wei Liu