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PLCL2 Polyclonal antibody

PLCL2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17421-1-AP

Synonyms

KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLC ε2, PLCE2, PLCL2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLCL2 Polyclonal antibody (17421-1-AP)

    WB analysis using 17421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17421-1-AP (PLCL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PLCL2 Polyclonal antibody (17421-1-AP)

    IF Staining of mouse brain using 17421-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 17421-1-AP (PLCL2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17421-1-AP targets PLCL2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCL2 fusion protein Ag11443
Full Name phospholipase C-like 2
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 126 kDa
GenBank Accession NumberBC036392
Gene Symbol PLCL2
Gene ID (NCBI) 23228
RRIDAB_2163732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 17421-1-AP antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

Protocols

Product Specific Protocols
WB protocol for PLCL2 antibody 17421-1-APDownload protocol
IF protocol for PLCL2 antibody 17421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Progesterone receptor isoform B regulates the Oxtr-Plcl2-Trpc3 pathway to suppress uterine contractility.

Authors - Mary C Peavey
View Article
mouseWB

Front Physiol

Release of GLP-1 and PYY in response to the activation of G protein-coupled bile acid receptor TGR5 is mediated by Epac/PLC-ε pathway and modulated by endogenous H2S.

Authors - Vanitha Bala
View Article