PLCL2 Polyclonal antibody

PLCL2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 17421-1-AP

Synonyms

KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLC ε2, PLCE2, PLCL2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

17421-1-AP targets PLCL2 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCL2 fusion protein Ag11443
Full Name phospholipase C-like 2
Calculated molecular weight 126 kDa
Observed molecular weight 126 kDa
GenBank accession numberBC036392
Gene symbol PLCL2
Gene ID (NCBI) 23228
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 17421-1-AP antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

Protocols

Product Specific Protocols
WB protocol for PLCL2 antibody 17421-1-APDownload protocol
IF protocol for PLCL2 antibody 17421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Physiol

Release of GLP-1 and PYY in response to the activation of G protein-coupled bile acid receptor TGR5 is mediated by Epac/PLC-ε pathway and modulated by endogenous H2S.

Authors - Vanitha Bala
mouseWB

Proc Natl Acad Sci U S A

Progesterone receptor isoform B regulates the Oxtr-Plcl2-Trpc3 pathway to suppress uterine contractility.

Authors - Mary C Peavey