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PLEK Monoclonal antibody

PLEK Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D5C2

Cat no : 66431-1-Ig

Synonyms

P47, Platelet 47 kDa protein, pleckstrin, PLEK



Tested Applications

Positive WB detected inHL-60 cells, pig spleen tissue, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66431-1-Ig targets PLEK in WB, IHC,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLEK fusion protein Ag3187
Full Name pleckstrin
Calculated molecular weight 350 aa, 40 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC018549
Gene symbol PLEK
Gene ID (NCBI) 5341
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Protocols

Product Specific Protocols
WB protocol for PLEK antibody 66431-1-IgDownload protocol
IHC protocol for PLEK antibody 66431-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols