POLR2D Antibody

POLR2D Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16093-1-AP

Synonyms

HSRBP4, HSRPB4, POLR2D, RNA polymerase II RPB4, RNA polymerase II subunit B4, RPB16



Tested Applications

Positive WB detected inHeLa cells, rat liver tissue, rat skeletal muscle tissue, MCF-7 cells, HepG2 cells
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

16093-1-AP targets POLR2D in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2D fusion protein Ag8701
Full Name polymerase (RNA) II (DNA directed) polypeptide D
Calculated molecular weight 142aa,16 kDa
Observed molecular weight 16-20 kDa
GenBank accession number BC017205
Gene symbol POLR2D
Gene ID (NCBI) 5433
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR2D antibody 16093-1-APDownload protocol
IP protocol for POLR2D antibody 16093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

chang wei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa S3
No image submitted with review