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POLR2H Polyclonal antibody

POLR2H Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15086-1-AP

Synonyms

hRPB8, hsRPB8, POLR2H, RPABC3, RPB17, RPB8, RPB8 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using POLR2H Polyclonal antibody (15086-1-AP)

    WB analysis of HL-60 using 15086-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using POLR2H Polyclonal antibody (15086-1-AP)

    WB analysis of MCF-7 using 15086-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using POLR2H Polyclonal antibody (15086-1-AP)

    WB analysis of rat liver using 15086-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using POLR2H Polyclonal antibody (15086-1-AP)

    WB analysis of mouse kidney using 15086-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using POLR2H Polyclonal antibody (15086-1-AP)

    WB analysis of mouse liver using 15086-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLR2H Polyclonal antibody (15086-1-AP)

    WB analysis of HeLa using 15086-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using POLR2H Polyclonal antibody (15086-1-AP)

    IP experiment of mouse liver using 15086-1-AP

    IP result of anti-POLR2H (IP:15086-1-AP, 4ug; Detection:15086-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using POLR2H Polyclonal antibody (15086-1-AP)

    IHC staining of human breast cancer using 15086-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using POLR2H Polyclonal antibody (15086-1-AP)

    IHC staining of human breast cancer using 15086-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLR2H Polyclonal antibody (15086-1-AP)

    IF Staining of HeLa using 15086-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15086-1-AP (POLR2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells, mouse kidney tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15086-1-AP targets POLR2H in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2H fusion protein Ag7166
Full Name polymerase (RNA) II (DNA directed) polypeptide H
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC000739
Gene Symbol POLR2H
Gene ID (NCBI) 5437
RRIDAB_2167826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR2H, also known as hRPB8, is a 150 amino acid protein, which localizes in nucleolus and is expressed in 228 organ(s), highest expression level in mucosa of transverse colon. POLR2H. POLR2H is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2H is a common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively

Protocols

Product Specific Protocols
WB protocol for POLR2H antibody 15086-1-APDownload protocol
IHC protocol for POLR2H antibody 15086-1-APDownload protocol
IF protocol for POLR2H antibody 15086-1-APDownload protocol
IP protocol for POLR2H antibody 15086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
View Article
humanIHC

Oncol Lett

Identification of the key genes and pathways in prostate cancer.

Authors - Shutong Fan
View Article
mouse

Genome Biol

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture

Authors - Yongpeng Jiang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Yuan (Verified Customer) (12-13-2019)

Worked for human Hela cell. Nice staining at nucleoli area.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Hela cell