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PPP1R9A Polyclonal antibody

PPP1R9A Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 18801-1-AP

Synonyms

KIAA1222, Neurabin 1, Neurabin I, NRB1, NRBI, PPP1R9A

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using PPP1R9A Polyclonal antibody (18801-1-AP)

    WB analysis of PC-3 using 18801-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18801-1-AP (PPP1R9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PPP1R9A Polyclonal antibody (18801-1-AP)

    WB analysis of PC-3 using 18801-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18801-1-AP (PPP1R9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R9A Polyclonal antibody (18801-1-AP)

    IF Staining of HepG2 using 18801-1-AP

    Immunofluorescent analysis of HepG2 cells, using PPP1R9A antibody 18801-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPP1R9A Polyclonal antibody (18801-1-AP)

    IF Staining of HeLa using 18801-1-AP

    Immunofluorescent analysis of Hela cells, using PPP1R9A antibody 18801-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inPC-3 cells
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18801-1-AP targets PPP1R9A in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 9A
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC150636
Gene Symbol PPP1R9A
Gene ID (NCBI) 55607
RRIDAB_10597849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R9A, also named as KIAA1222 and NEB1, binds to actin filaments (F-actin) and shows cross-linking activity. PPP1R9A has been found to be concentrated in the synapse of developed neurons and in the lamellipodia of the growth cone during neuron development.PPP1R9A may be involved in neurite formation. PPP1R9A inhibits protein phosphatase 1-alpha activity.

Protocols

Product Specific Protocols
WB protocol for PPP1R9A antibody 18801-1-APDownload protocol
IF protocol for PPP1R9A antibody 18801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols