PPP2R2C Polyclonal antibody

PPP2R2C Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 12747-1-AP

Synonyms

B55 GAMMA, IMYPNO, IMYPNO1, PP2A subunit B isoform gamma, PPP2R2C, PR52, PR55G



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12747-1-AP targets PPP2R2C in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R2C fusion protein Ag3470
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
Calculated molecular weight 430 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC032954
Gene symbol PPP2R2C
Gene ID (NCBI) 5522
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP2R2C antibody 12747-1-APDownload protocol
IHC protocol for PPP2R2C antibody 12747-1-APDownload protocol
FC protocol for PPP2R2C antibody 12747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biochem

Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.

Authors - Shunta Ikeda
human

J Cell Sci

The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.

Authors - Liping Chen