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PPP2R2C Polyclonal antibody

PPP2R2C Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 12747-1-AP

Synonyms

B55 GAMMA, IMYPNO, IMYPNO1, PP2A subunit B isoform gamma, PPP2R2C, PR52, PR55G

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PPP2R2C Polyclonal antibody (12747-1-AP)

    WB analysis of HeLa using 12747-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PPP2R2C Polyclonal antibody (12747-1-AP)

    WB analysis of mouse testis using 12747-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PPP2R2C Polyclonal antibody (12747-1-AP)

    WB analysis of MCF-7 using 12747-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPP2R2C Polyclonal antibody (12747-1-AP)

    IHC staining of human breast cancer using 12747-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPP2R2C Polyclonal antibody (12747-1-AP)

    IHC staining of human breast cancer using 12747-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PPP2R2C Polyclonal antibody (12747-1-AP)

    IHC staining of human brain using 12747-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PPP2R2C Polyclonal antibody (12747-1-AP)

    IHC staining of human brain using 12747-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP2R2C Polyclonal antibody (12747-1-AP)

    IHC staining of human testis using 12747-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP2R2C Polyclonal antibody (12747-1-AP)

    IHC staining of human testis using 12747-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of MCF-7 cells using PPP2R2C Polyclonal antibody (12747-1-AP)

    FC experiment of MCF-7 using 12747-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug PPP2R2C antibody (12747-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12747-1-AP targets PPP2R2C in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R2C fusion protein Ag3470
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
Calculated Molecular Weight 430 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC032954
Gene Symbol PPP2R2C
Gene ID (NCBI) 5522
RRIDAB_2170099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP2R2C antibody 12747-1-APDownload protocol
IHC protocol for PPP2R2C antibody 12747-1-APDownload protocol
FC protocol for PPP2R2C antibody 12747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

J Cell Sci

The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.

Authors - Liping Chen
View Article
mouseWB

J Biochem

Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.

Authors - Shunta Ikeda
View Article
mouseWB

Biochim Biophys Acta Mol Cell Res

Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport

Authors - G F Sengül
View Article