Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
12747-1-AP targets PPP2R2C in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3470 Product name: Recombinant human PPP2R2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 131-430 aa of BC032954 Sequence: LQVPVLKPMDLMVEVSPRRIFANGHTYHINSISVNSDCETYMSADDLRINLWHLAITDRSFNIVDIKPANMEDLTEVITASEFHPHHCNLFVYSSSKGSLRLCDMRAAALCDKHSKLFEEPEDPSNRSFFSEIISSVSDVKFSHSGRYMLTRDYLTVKVWDLNMEARPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSDSVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAVLKPRRVCVGGKRRRDDISVDSLDFTKKILHTAWHPAENIIAIAATNNLYIFQDKVNSDMH Predict reactive species |
| Full Name | protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform |
| Calculated Molecular Weight | 430 aa, 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC032954 |
| Gene Symbol | PPP2R2C |
| Gene ID (NCBI) | 5522 |
| RRID | AB_2170099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2T4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein phosphatase 2 regulatory subunit B gamma, also known as PPP2R2C or B55-gamma, encodes a gamma isoform of the subunit B55 subfamily, which is a regulatory subunit of Protein phosphatase type 2A (PP2A) (PMID: 24126060). PPP2R2C has been identified as a potential tumor suppressor in several cancers, including prostate and brain cancers (PMID: 23493267, PMID: 24126060). The decreased expression of PPP2R2C is associated with tumor progression, metastasis, and poorer patient outcomes in prostate cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP2R2C antibody 12747-1-AP | Download protocol |
| WB protocol for PPP2R2C antibody 12747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice | ||
J Biochem Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell. | ||
Biochim Biophys Acta Mol Cell Res Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport | ||
Toxicol Appl Pharmacol Chronic arsenic exposure suppresses ATM pathway activation in human keratinocytes | ||
Cell Signal METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression |
