PR Monoclonal antibody

PR Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D1D12

Cat no : 66300-1-Ig

Synonyms

NR3C3, PGR, PR, progesterone receptor



Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Sample-dependent, check data in validation data gallery

Product Information

66300-1-Ig targets PR in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PR fusion protein Ag22992
Full Name progesterone receptor
Calculated molecular weight 933 aa, 99 kDa
GenBank accession numberNM_000926
Gene symbol PGR
Gene ID (NCBI) 5241
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Protocols

Product Specific Protocols
IHC protocol for PR antibody 66300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols