Biotin-conjugated PR Monoclonal antibody

PR Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

3D1D12

Cat no : Biotin-66300

Synonyms

NR3C3, PGR, PR, progesterone receptor

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Validation Data Gallery

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  IHC staining of human breast cancer using Biotin-66300

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using Biotin-66300

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using Biotin-66300

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66300 targets PR in IHC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PR fusion protein Ag22992
• Full Name: progesterone receptor
• Calculated Molecular Weight: 933 aa, 99 kDa
• GenBank Accession Number: NM_000926
• Gene Symbol: Progesterone Receptor
• Gene ID (NCBI): 5241
• RRID: AB_2883069
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.