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  • KD/KO Validated

PKC alpha Antibody

PKC alpha Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21991-1-AP

Synonyms

AAG6, PKC A, PKC alpha, PKCA, PKCα, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, NIH/3T3 cells, rat kidney tissue, human peripheral blood platelets
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21991-1-AP is PKC alpha Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC alpha fusion protein Ag17275
Full Name protein kinase C, alpha
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberAK055431
Gene symbol PRKCA
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Protocols

Product Specific Protocols
WB protocol for PKC alpha antibody 21991-1-APDownload protocol
IHC protocol for PKC alpha antibody 21991-1-APDownload protocol
IF protocol for PKC alpha antibody 21991-1-APDownload protocol
IP protocol for PKC alpha antibody 21991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Physiol

Sensory plasticity of carotid body is correlated with oxidative stress in paraventricular nucleus during chronic intermittent hypoxia.

Authors - Pei Liu
mouseWB

Int J Mol Sci

Silencing of Transcription Factor Sp1 Promotes SN1 Transporter Regulation by Ammonia in Mouse Cortical Astrocytes.

Authors - Katarzyna Dąbrowska
mouseIF

Cell Death Dis

Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma.

Authors - Hiroki Sano
humanWB

Invest New Drugs

PMID: 31124054

Authors - Qing-Hua Liu
humanWB

Intractable Rare Dis Res

A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells.

Authors - Liang Jiang
mouseIF

Invest Ophthalmol Vis Sci

Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Authors - Kei Akaiwa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Samantha (Verified Customer) (02-24-2020)

Bovine vascular smooth muscle cells were transfected with either control siRNA or PKCalpha siRNA for up to 7 days, with transfections repeated every 48-72 hours. Blocking agent: 5% (w/v) milk in TBST for 1hr Primary antibody diluted in 5% (w/v) milk in TBST; incubated overnight at 4 degrees.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Bovine vascular smooth muscle cells
PKC alpha Antibody Western Blot, validation (1:750 dilution) in Bovine vascular smooth muscle cells (Cat no:21991-1-AP)
FH

Ryan (Verified Customer) (01-10-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina
PKC alpha Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:21991-1-AP)