PKC Delta Polyclonal antibody
PKC Delta Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 19132-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HT-1080 cells, Jurkat cells, MCF-7 cells, mouse colon tissue |
Positive IP detected in | A431 cells |
Positive IHC detected in | human brain tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
The immunogen of 19132-1-AP is PKC Delta Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PKC Delta fusion protein Ag5631 |
Full Name | protein kinase C, delta |
Calculated molecular weight | 78 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC043350 |
Gene symbol | PRKCD |
Gene ID (NCBI) | 5580 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PKC Delta antibody 19132-1-AP | Download protocol |
IHC protocol for PKC Delta antibody 19132-1-AP | Download protocol |
IP protocol for PKC Delta antibody 19132-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Neurochem Int Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes. | ||
Food Chem Toxicol G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes. |