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PKC Delta Polyclonal antibody

PKC Delta Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19132-1-AP

Synonyms

MAY1, PKC delta, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

Validation Data Gallery

  • WB analysis of A431 using 19132-1-AP

    WB analysis of A431 using 19132-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19132-1-AP

    IHC staining of human brain using 19132-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 19132-1-AP

    IHC staining of human brain using 19132-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A431 using 19132-1-AP

    IP experiment of A431 using 19132-1-AP

    IP Result of anti-PKC delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug.

Tested Applications

Positive WB detected inA431 cells, HT-1080 cells, Jurkat cells, MCF-7 cells, mouse colon tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 19132-1-AP is PKC Delta Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Delta fusion protein Ag5631
Full Name protein kinase C, delta
Calculated molecular weight 78 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC043350
Gene symbol PRKCD
Gene ID (NCBI) 5580
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Delta antibody 19132-1-APDownload protocol
IHC protocol for PKC Delta antibody 19132-1-APDownload protocol
IP protocol for PKC Delta antibody 19132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurochem Int

Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes.

Authors - Katarzyna Dąbrowska
View Article
ratWB

Food Chem Toxicol

G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.

Authors - Yao Liu
View Article