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  • KD/KO Validated

PRMT1 Polyclonal antibody

PRMT1 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11279-1-AP

Synonyms

ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1



Tested Applications

Positive WB detected inA549 cells, HepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

11279-1-AP targets PRMT1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT1 fusion protein Ag1790
Full Name protein arginine methyltransferase 1
Calculated molecular weight 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC019268
Gene symbol PRMT1
Gene ID (NCBI) 3276
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRMT1 antibody 11279-1-APDownload protocol
IHC protocol for PRMT1 antibody 11279-1-APDownload protocol
IF protocol for PRMT1 antibody 11279-1-APDownload protocol
FC protocol for PRMT1 antibody 11279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncogene

PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.

Authors - Xin-Ke Yin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs