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PSMD4 Polyclonal antibody

PSMD4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14899-1-AP

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, Raji cells, rat spleen tissue, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14899-1-AP targets PSMD4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD4 fusion protein Ag6691
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular weight 41 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC002365
Gene symbol PSMD4
Gene ID (NCBI) 5710
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Protocols

Product Specific Protocols
WB protocol for PSMD4 antibody 14899-1-APDownload protocol
IHC protocol for PSMD4 antibody 14899-1-APDownload protocol
IF protocol for PSMD4 antibody 14899-1-APDownload protocol
FC protocol for PSMD4 antibody 14899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virology

Interaction of HPV E6 oncoproteins with specific proteasomal subunits.

Authors - Tomai? Vjekoslav V
humanWB

Biochim Biophys Acta Mol Basis Dis

Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.

Authors - Diego Sbardella
humanWB

J Biol Chem

Chaperone-mediated 26S Proteasome Remodeling Facilitates Free K63 Ubiquitin Chain Production and Aggresome Clearance.

Authors - Priyaanka Nanduri
  • KD Validated
humanWB

Mol Cell Proteomics

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Authors - Tatjana Goebel
humanWB

J Cell Sci

S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.

Authors - Zheng Wang
humanWB

Oncogene

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A