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PTAFR Polyclonal antibody

PTAFR Polyclonal Antibody for FC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 20921-1-AP

Synonyms

PAF R, PAFR, PTAFR

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung tissue using PTAFR Polyclonal antibody (20921-1-AP)

    IHC staining of human lung using 20921-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20921-1-AP (PTAFR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PTAFR Polyclonal antibody (20921-1-AP)

    IHC staining of human lung using 20921-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20921-1-AP (PTAFR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PTAFR Polyclonal antibody (20921-1-AP)

    IHC staining of human heart using 20921-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20921-1-AP (PTAFR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PTAFR Polyclonal antibody (20921-1-AP)

    IHC staining of human heart using 20921-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20921-1-AP (PTAFR Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HL-60 cells using PTAFR Polyclonal antibody (20921-1-AP)

    FC experiment of HL-60 using 20921-1-AP

    1X10^6 HL-60 cells were stained with 0.2ug PTAFR antibody (20921-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive IHC detected inhuman lung tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHL-60 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20921-1-AP is PTAFR Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTAFR fusion protein Ag15128
Full Name platelet-activating factor receptor
Calculated Molecular Weight 342 aa, 39 kDa
GenBank Accession NumberBC013816
Gene Symbol PTAFR
Gene ID (NCBI) 5724
RRIDAB_2878766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for PTAFR antibody 20921-1-APDownload protocol
FC protocol for PTAFR antibody 20921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Struct Mol Biol

Structural basis for signal recognition and transduction by platelet-activating-factor receptor.

Authors - Can Cao
View Article
mouseWB

Gut Pathog

Virulence factors and mechanisms of paediatric pneumonia caused by Enterococcus faecalis

Authors - Zhiying Tian
View Article