|Positive WB detected in||fetal human brain tissue, RAW 264.7 cells, DU 145 cells, HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells|
|Positive IHC detected in||human breast hyperplasia tissue, human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1200|
|Sample-dependent, check data in validation data gallery|
60300-1-Ig targets PTEN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||PTEN fusion protein Ag17274|
|Full Name||phosphatase and tensin homolog|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC005821|
|Gene ID (NCBI)||5728|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.
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