PTEN Antibody

PTEN Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5C10B6

Cat no : 60300-1-Ig

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1



Tested Applications

Positive WB detected infetal human brain tissue, RAW 264.7 cells, DU 145 cells, HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1200
Sample-dependent, check data in validation data gallery

Product Information

60300-1-Ig targets PTEN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PTEN fusion protein Ag17274
Full Name phosphatase and tensin homolog
Calculated molecular weight 47 kDa
Observed molecular weight 55 kDa
GenBank accession number BC005821
Gene symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

Protocols

Product Specific Protocols
WB protocol for PTEN antibody 60300-1-IgDownload protocol
IHC protocol for PTEN antibody 60300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Obstet Gynecol Reprod Biol

Comprehensive assessment the expression of core elements related to IGFIR/PI3K pathway in granulosa cells of women with polycystic ovary syndrome.

Authors - Tingting He
humanWB,IF

Evid Based Complement Alternat Med

Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.

Authors - Yubo Guo
humanWB,IF

BMC Complement Med Ther

Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

Authors - Li Han
humanIHC

Oncol Lett

Chemoresistance is associated with Beclin-1 and PTEN expression in epithelial ovarian cancers.

Authors - Huanchun Ying
humanWB

Dig Dis Sci

LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.

Authors - Jianhui Liu
mouseWB

Biochem Biophys Res Commun

LSD1 negatively regulates autophagy in myoblast cells by driving PTEN degradation.

Authors - Ying-Xu Shi