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PTH2R Polyclonal antibody

PTH2R Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 14166-1-AP

Synonyms

parathyroid hormone 2 receptor, PTH2 receptor, PTH2R, PTHR2

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of L02 using 14166-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of mouse testis using 14166-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of mouse liver using 14166-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of BxPC-3 using 14166-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of mouse pancreas using 14166-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PTH2R Polyclonal antibody (14166-1-AP)

    WB analysis of human testis using 14166-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human pancreas cancer using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human pancreas cancer using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PTH2R Polyclonal antibody (14166-1-AP)

    IHC staining of human renal cell carcinoma using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of BxPC-3 cells using PTH2R Polyclonal antibody (14166-1-AP)

    FC experiment of BxPC-3 using 14166-1-AP

    1X10^6 BxPC-3 cells were stained with 0.2ug PTH2R antibody (14166-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inL02 cells, BxPC-3 cells, human testis tissue, mouse liver tissue, mouse pancreas tissue, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14166-1-AP targets PTH2R in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTH2R fusion protein Ag5364
Full Name parathyroid hormone 2 receptor
Calculated Molecular Weight 550 aa, 62 kDa
Observed Molecular Weight 62-66 kDa
GenBank Accession NumberBC036811
Gene Symbol PTH2R
Gene ID (NCBI) 5746
RRIDAB_2253212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTH2R antibody 14166-1-APDownload protocol
IHC protocol for PTH2R antibody 14166-1-APDownload protocol
FC protocol for PTH2R antibody 14166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols