|Positive WB detected in||HeLa cells, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13008-1-AP targets PTPRS in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PTPRS fusion protein Ag3691|
|Full Name||protein tyrosine phosphatase, receptor type, S|
|Calculated molecular weight||128aa,14 kDa; 1948aa,217 kDa|
|Observed molecular weight||75-100 kDa|
|GenBank accession number||BC029496|
|Gene ID (NCBI)||5802|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Type II a receptor protein tyrosine phosphatases (rPTPσ) are cell surface receptors important for nervous system development, function, and repair.The expression of rPTPσ has previously been reported in b-cells and other target organs for INS although the probes chosen did not permit to distinguish between the splice variants.Proteolytic processing near the transmembrane domain generates an extracellular N-terminal E-domain of 130 kDa and a C-terminal P-domain of approximately 85 kDa of rPTPσ,and the short splice variants rPTPσ 3 and 4 contain an E-domain of 95 kDa (PMID: 16552719). rPTPσ expression was observed in tissue lysates of the adult mouse sensory-motor cortex and thoracic spinal cord (T8-T10) as a 75-80kDa immunoreactive band (PMID: 19780196).
|Product Specific Protocols|
|WB protocol for PTPRS antibody 13008-1-AP||Download protocol|
|IHC protocol for PTPRS antibody 13008-1-AP||Download protocol|
|IP protocol for PTPRS antibody 13008-1-AP||Download protocol|
|FC protocol for PTPRS antibody 13008-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Biochem
Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.
Curr Eye Res
Expression of Perineuronal Nets, Parvalbumin and Protein Tyrosine Phosphatase σ in the Rat Visual Cortex During Development and After BFD.
J Gene Med
Downregulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR-455-5p.
Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.