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PTPRS Polyclonal antibody

PTPRS Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 13008-1-AP

Synonyms

PTPRS, PTPSIGMA, R PTP S, R PTP sigma, rPTPσ



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13008-1-AP targets PTPRS in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRS fusion protein Ag3691
Full Name protein tyrosine phosphatase, receptor type, S
Calculated molecular weight128aa,14 kDa; 1948aa,217 kDa
Observed molecular weight 75-100 kDa
GenBank accession numberBC029496
Gene symbol PTPRS
Gene ID (NCBI) 5802
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type II a receptor protein tyrosine phosphatases (rPTPσ) are cell surface receptors important for nervous system development, function, and repair.The expression of rPTPσ has previously been reported in b-cells and other target organs for INS although the probes chosen did not permit to distinguish between the splice variants.Proteolytic processing near the transmembrane domain generates an extracellular N-terminal E-domain of 130 kDa and a C-terminal P-domain of approximately 85 kDa of rPTPσ,and the short splice variants rPTPσ 3 and 4 contain an E-domain of 95 kDa (PMID: 16552719). rPTPσ expression was observed in tissue lysates of the adult mouse sensory-motor cortex and thoracic spinal cord (T8-T10) as a 75-80kDa immunoreactive band (PMID: 19780196).

Protocols

Product Specific Protocols
WB protocol for PTPRS antibody 13008-1-APDownload protocol
IHC protocol for PTPRS antibody 13008-1-APDownload protocol
IP protocol for PTPRS antibody 13008-1-APDownload protocol
FC protocol for PTPRS antibody 13008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

J Cell Biochem

Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Authors - Jun Zhong
ratWB,IHC

Curr Eye Res

Expression of Perineuronal Nets, Parvalbumin and Protein Tyrosine Phosphatase σ in the Rat Visual Cortex During Development and After BFD.

Authors - Liu Hui H
humanWB

J Gene Med

Downregulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR-455-5p.

Authors - Jingjing Wu
mouseWB

Elife

Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.

Authors - Andrea M Gomez