|Positive WB detected in||HeLa cells, L02 cells, Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1500-1:6000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17146-1-AP targets PYCR2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||African green monkey, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PYCR2 fusion protein Ag10918|
|Full Name||pyrroline-5-carboxylate reductase family, member 2|
|Calculated molecular weight||320 aa, 34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC020553|
|Gene ID (NCBI)||29920|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.
|Product Specific Protocols|
|WB protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|IHC protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|IF protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|IP protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (07-03-2020)
Giving a very sharp and clean result