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  • KD/KO Validated

PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17146-1-AP

Synonyms

P5C reductase 2, P5CR 2, P5CR2, PYCR2



Tested Applications

Positive WB detected inHeLa cells, L02 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17146-1-AP targets PYCR2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR2 fusion protein Ag10918
Full Name pyrroline-5-carboxylate reductase family, member 2
Calculated molecular weight 320 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC020553
Gene symbol PYCR2
Gene ID (NCBI) 29920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR2 antibody 17146-1-APDownload protocol
IHC protocol for PYCR2 antibody 17146-1-APDownload protocol
IF protocol for PYCR2 antibody 17146-1-APDownload protocol
IP protocol for PYCR2 antibody 17146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging Dis

A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.

Authors - Yu-Wen Huang
humanWB

Nat Commun

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Authors - Ling Guo
humanWB

Am J Respir Cell Mol Biol

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Authors - Robert B Hamanaka
humanWB

Sci Rep

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Authors - Bobby Jones
humanWB

Sci Rep

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Authors - Wei Liu
  • KD Validated
humanWB

Cell Rep

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

Authors - Kate E R Hollinshead
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (07-03-2020)

Giving a very sharp and clean result

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human