|Positive WB detected in||HeLa cells, L02 cells, Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1500-1:6000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17146-1-AP targets PYCR2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PYCR2 fusion protein Ag10918|
|Full Name||pyrroline-5-carboxylate reductase family, member 2|
|Calculated molecular weight||320 aa, 34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC020553|
|Gene ID (NCBI)||29920|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.
|Product Specific Protocols|
|WB protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|IHC protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|IF protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|IP protocol for PYCR2 antibody 17146-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (07-03-2020)
Giving a very sharp and clean result