PYGL Polyclonal antibody

PYGL Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 55429-1-AP

Synonyms

GSD6, phosphorylase, glycogen, liver, PYGL



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55429-1-AP targets PYGL in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphorylase, glycogen, liver
Calculated molecular weight 97 kDa
Observed molecular weight 97-105 kDa
GenBank accession numberNM_002863
Gene symbol PYGL
Gene ID (NCBI) 5836
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PYGL belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). This antibody is specific to PYGL. It has no cross reaction to PYGM and PYGB.

Protocols

Product Specific Protocols
WB protocol for PYGL antibody 55429-1-APDownload protocol
IHC protocol for PYGL antibody 55429-1-APDownload protocol
IF protocol for PYGL antibody 55429-1-APDownload protocol
IP protocol for PYGL antibody 55429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols