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Tested Applications
| Positive WB detected in | EGF treated A431 cells |
| Positive IF/ICC detected in | hEGF treated A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86398-1-RR targets Phospho-Tyrosine in WB, IF/ICC, ELISA applications and shows reactivity with - samples.
| Tested Reactivity | - |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Phospho-Tyrosine |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phospho-Tyrosine antibody refers to a class of pan-specific antibodies or detection reagents that recognize all phosphorylated tyrosine residues regardless of the surrounding amino-acid sequence. These tools are widely used to monitor global tyrosine phosphorylation events in signal-transduction studies, cancer research and drug-discovery screens.
This antibody does not cross-react with peptides containing Phospho-Serine or Phospho-Threonine.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-Tyrosine antibody 86398-1-RR | Download protocol |
| WB protocol for Phospho-Tyrosine antibody 86398-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
