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Phospho-Tyrosine Recombinant monoclonal antibody

Phospho-Tyrosine Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 86398-1-RR
Clone No.250495G7
Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

- and More (1)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

P Tyr, p Tyrosine, pan phospho, pan-phospho, Pan-phospho-Tyr

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Freight/Packing: -

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  • Western Blot (WB) analysis of various lysates using Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR)

    WB analysis using 86398-1-RR

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 86398-1-RR (Phospho-Tyrosine antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR)

    IF Staining of A431 using 86398-1-RR

    Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR)

    IF Staining of A431 using 86398-1-RR

    Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Flow cytometry (FC) experiment of A431 cells using Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR)

    FC experiment of A431 using 86398-1-RR

    1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR, Clone:250495G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Tested Applications

Positive WB detected inEGF treated A431 cells
Positive IF/ICC detected inhEGF treated A431 cells
Positive FC (Intra) detected inEGF treated A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86398-1-RR targets Phospho-Tyrosine in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with - samples.

Tested Reactivity -
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name Phospho-Tyrosine
Gene Symbol
Gene ID (NCBI)
RRIDAB_3744861
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospho-Tyrosine antibody refers to a class of pan-specific antibodies or detection reagents that recognize all phosphorylated tyrosine residues regardless of the surrounding amino-acid sequence. These tools are widely used to monitor global tyrosine phosphorylation events in signal-transduction studies, cancer research and drug-discovery screens.
This antibody does not cross-react with peptides containing Phospho-Serine or Phospho-Threonine.

Protocols

Product Specific Protocols
FC protocol for Phospho-Tyrosine antibody 86398-1-RRDownload protocol
IF protocol for Phospho-Tyrosine antibody 86398-1-RRDownload protocol
WB protocol for Phospho-Tyrosine antibody 86398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

SpeciesApplicationTitle
mouseIP, WB

Neuropharmacology

Cyclic neuronostatin regulates glucose homeostasis and food intake through GPR107 phosphorylation.

Authors - Yanhong Li
View Article
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